• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>SPIE Conference on Biomedical Applications in Molecular, Structural, and Functional Imaging >DETECTING METASTASIS OF GASTRIC CARCINOMA USING HIGH-RESOLUTIONMICRO-CT SYSTEM: IN VIVO SMALL ANIMAL STUDY
【24h】

DETECTING METASTASIS OF GASTRIC CARCINOMA USING HIGH-RESOLUTIONMICRO-CT SYSTEM: IN VIVO SMALL ANIMAL STUDY

机译：使用高分辨率含镉系统检测胃癌转移：体内小动物研究

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Immunocytochemical and immunofluorescence staining are used for identifying the characteristics of metastasis in traditional ways. Micro-computed tomography (micro-CT) is a useful tool for monitoring and longitudinal imaging of tumor in small animal in vivo. In present study, we evaluated the feasibility of the detection for metastasis of gastric carcinoma by high-resolution micro-CT system with omnipaque accumulative enhancement method in the organs. Firstly, a high-resolution micro-CT ZKKS-MCT-sharp micro-CT was developed by our research group and Guangzhou Zhongke Kaisheng Medical Technology Co., Ltd. Secondly, several gastric carcinoma models were established through inoculating 2x106 BGC-823 gastric carcinoma cells subcutaneously. Thirdly, micro-CT scanning was performed after accumulative enhancement method of intraperitoneal injection of omnipaque contrast agent containing 360 mg iodine with a concentration of 350 mg I/ml. Finally, we obtained high-resolution anatomical information of the metastasis in vivo in a BALB/c NuNu nude mouse, the 3D tumor architecture is revealed in exquisite detail at about 35 pm spatial resolution. In addition, the accurate shape and volume of the micrometastasis as small as 0.78 mm3 can be calculated with our software. Overall, our data suggest that this imaging approach and system could be used to enhance the understanding of tumor proliferation, metastasis and could be the basis for evaluating anti-tumor therapies.
机译：免疫细胞化学和免疫荧光染色用于以传统方式鉴定转移的特征。微型计算机断层扫描（Micro-CT）是用于在体内小动物中肿瘤监测和纵向成像的有用工具。在目前的研究中，我们通过在器官中具有高分辨率微型CT系统，通过高分辨率微型CT系统评估了胃癌转移的可行性。首先，通过我们的研究组和广州中信kaisheng医疗技术有限公司开发了高分辨率的微型CT ZKKS-MCT-Sharp Micro-CT。通过接种2x106 BGC-823胃癌，建立了几种胃癌模型的胃癌模型皮下细胞。第三，在腹腔内注射含有360mg碘的腹腔内注射含有360mg I / ml的浓度的含有360mg碘的累积增强方法之后进行微型CT扫描。最后，我们在BALB / C Nunu裸鼠中获得了体内转移的高分辨率解剖学信息，3D肿瘤架构在大约35点的空间分辨率下揭示了精致的细节。此外，可以通过我们的软件计算小于0.78mm3的微转移的精确形状和体积。总体而言，我们的数据表明，这种成像方法和系统可用于增强对肿瘤增殖，转移的理解，并且可以是评估抗肿瘤疗法的基础。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号