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首页> 外文会议>International Rubus and Ribes Symposium >Cryopreservation In Vitro of Blackberry '?a?anska Bestrna' Shoot Tips by Encapsulation Dehydration
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Cryopreservation In Vitro of Blackberry '?a?anska Bestrna' Shoot Tips by Encapsulation Dehydration

机译：黑莓'a？a？anska bestrna'拍摄提示的冷冻保存，通过封装脱水

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In the present study, in vitro grown shoot tips of blackberry (Rubus subg. Rubus Watson.) '?a?anska Bestrna' were tested for regrowth after cryopreservation using slightly modified encapsulation dehydration method described by Dereuddre et al. (1990). Apical shoot tips 2-3 mm length were encapsulated in alginate beads composed of 3, 5 and 10% (w/v) alginic acid sodium salt in calcium-free liquid MS medium supplemented with 1 mg L~(-1) BA, 0.1 mg L~(-1) IBA and 0.1 mg L~(-1) GA_3. Polymerization was done in liquid MS medium with 100 mM CaCl_2 for 30 min at room temperature. Encapsulated shoot tips were pre-treated in liquid MS medium with 0.75 or 1 M sucrose for 24 h in growth room and dehydrated for 4 and 8 h to 29 and 20% moisture content, resp. (fresh weight basis) before rapid immersion in liquid nitrogen (LN) for 1 h. Upon thawing performed by placing the cryovials in the air currant of the laminar airflow cabinet for 2 min the beads were directly transferred on a regrowth medium. Explants showing normal development 28 days after bead burst were considered be regrowing. The osmotic dehydration in 1 M sucrose followed by 8 h of desiccation gave the highest survival rate (22.2%) and regrowth (16.7%) after freezing in LN for explants encapsulated in 5% alginate beads. Cryopreserved shoot tips multiplied in the three subcultures had normal morphology with similar multiplication capacity in comparison with non- cryopreserved shoots.
机译：在目前的研究中，使用Dereuddre等人描述的微微修饰的封装脱水方法在冷冻保存后进行了体外生长的射击尖端（Rubus Subg。）'？a？a？anska bestrna'进行再生进行再生进行再生。 （1990）。 2-3mm长度的顶端射击尖端在含有3,5和10％（w / v）藻酸钠盐中的藻酸盐珠粒中包封，所述钙液Ms培养基中的3,5和10％（w / v）藻酸钠盐，其补充有1mg l〜（-1）ba，0.1 Mg L〜（-1）IBA和0.1mg L〜（-1）GA_3。在室温下在100mM CaCl_2的液态MS培养基中在液态MS培养基中进行聚合30分钟。将封装的芽尖在液态MS培养基中预处理，在生长室中为0.75或1M蔗糖24小时，并脱水4和8小时至29％至20％的水分含量。 （鲜重基础）在快速浸入液氮（LN）之前1小时。在通过将阴道的缓和在层流气流柜中的空气摩尔人中进行了2分钟进行的解冻时，将珠子直接转移在再生培养基上。在珠爆后28天显示正常发育的外植体被认为是重生。 1 m蔗糖中的渗透脱水，然后在8小时内冻结后的8小时，并在冻融膜中冻结在5％藻酸盐珠粒中的植物中冷冻后的生存率（22.2％）和再生（16.7％）。与非冷冻保存的芽相比，三个潜水次繁殖的射击提示乘以相似的乘法能力。

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