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Detection of toxic chromium species in water using cell-based sensor systems

机译：基于细胞的传感器系统检测水中有毒铬物种

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In this study, several cell-based sensor systems have been evaluated and compared for their usability as environmental sensors in aquatic systems. Toxic chromium(VI) ions as well as non-toxic chromium(III) ions have been used as model substances, since chromium pollutions are of major concern. The investigated systems perform impedance, acidification and oxygen consumption measurements as well as combinations of these parameters. With multi-parametric sensor systems, the detection of Cr(VI) contamination is possible even at the limit of drinking water regulations (0.5 μM Cr(VI)). Oxygen consumption seems to be the most sensitive parameter as it increases at lowest concentrations within 3 hours. The continuous recording of physiologically relevant data even shows the early beginning of a toxic reaction. Tested V79 hamster lung fibroblast cells react only towards the presence of toxic Cr(VI), while non toxic Cr(lll) is not impairing the cellular physiology or morphology. The use of living cells in combination with sensor technology is promising to have a major impact in the field of broad-band environmental sensors.
机译：在这项研究中，已经评估了几个基于电池的传感器系统，并将其与水生系统中的环境传感器的可用性进行了比较。由于铬污染有着重大关注，因此已用作模型物质的有毒铬（VI）离子以及无毒铬（III）离子。调查系统执行阻抗，酸化和氧气消耗测量以及这些参数的组合。利用多参数传感器系统，即使在饮用水法规（0.5μmCr（VI））的极限下，也可以检测Cr（VI）污染。氧气消耗似乎是最敏感的参数，因为它在3小时内以最低浓度增加。生理相关数据的连续记录甚至显示毒性反应的早期开始。测试的V79仓鼠肺成纤维细胞仅在毒性Cr（VI）的存在下反应，而无毒Cr（LLL）不会损害细胞生理学或形态。使用活细胞与传感器技术的使用是有希望在宽带环境传感器领域产生重大影响。

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