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Comet assay determine DNA damage baseline level of Mollusca hemolymph cells in Poyang Lake and surrounding waters

机译:Comet测定确定鄱阳湖和周边水域Mollusca血淋巴细胞的DNA损伤基线水平

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Mollusc species were sampled in different sites based on pollution level in Poyang lake and its surrounding waters during withered water period (from Oct to Dec) and plentiful water period (from May to June) from 2007 to 2008. Single-cell gel electrophoresis (SCGE) was used to analyze their haemolymph cells Arbitrary Unit(AU) and Olive tailmoment (OTM) value of DNA damage in molluca. The results showed that the mean DNA damage value Jin river>Nanxin>Xiu stream in withered water period; and in Plentiful water period, Hukou>Qinglan lake>Xiyuan. Two mollusc species Corbicula fluminea and Bellamya aeruginosa have higher DNA damage value than other mussel in all sites. Comparing the DNA damage value of Bellamya aeruginosa collected in twenty sites in 2008, the highest DNA damage value was observed in Dacha lake and lowest in Xiyuan. Meanwhile, the highest DNA damage value of Unio douglasiae was recorded Chucha river from ten sites, and lowest in Xiyuan river.
机译:在2007年至2008年,基于鄱阳湖及其周围水域(从5月至6月起从5月至6月)的污染水平,在鄱阳湖及其周边水域(从5月至6月)的不同部位进行了取样。单细胞凝胶电泳(SCGE )用于分析Molluca中的DNA损伤的毒性吡喃蜂氨酸任意单位(Au)和橄榄尾剂(OTM)值。结果表明,平均DNA损伤价值金河>南新植物>富含水分的水分;而在丰富的水时期,户口>清澜湖>西苑。两种软体动物物种Corbicula Flumuea和Bellamya铜绿假单胞菌的DNA损伤率高于所有网站中的其他贻贝。比较2008年二十个遗址收集的Bellamya铜绿假单胞菌的DNA损伤值,在达雅湖中观察到最高的DNA损伤值,并在西苑最低。同时,UIO Douglasiae的最高DNA损伤价值从十个地点记录了Chucha河,最低的西苑河最低。

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