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首页> 外文会议>International Conference on Energy, Environment and Sustainable Development >Fuel ethanol production from lignocellulosic biomass using a recombinant yeast strain
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Fuel ethanol production from lignocellulosic biomass using a recombinant yeast strain

机译：使用重组酵母菌菌株从木质纤维素生物质产生燃料乙醇

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Fuel ethanol production from lignocellulosic biomass corn stover was investigated. Compared with acid pretreatment and ammonia steeping pretreatment, alkali pretreatment with 2% NaOH markedly enhanced lignin removal and thereby improved the enzymatic hydrolysis yield to 81.2% by 48 h. Fed-batch hydrolysis was started with a batch hydrolysis containing 80 g/l substrate, with cellulosic residue added at 6 and 12 h twice to get a final substrate concentration of 110 g/l. After 72 h of hydrolysis, the reducing sugar concentration reached 89.5 g/l with a hydrolysis yield of 83.3%. Further fermentation of the cellulosic hydrolysate containing 56.7 g/l glucose and 23.6 g/l xylose was performed using a recombinant yeast strain Saccharomyces cerevisiae ZU-10, and 36.3 g/l ethanol was produced within 72 h. The research results are meaningful in fuel ethanol production from renewable lignocellulosic biomass
机译：研究了木质纤维素生物量玉米秸秆的燃料乙醇生产。与酸预处理和氨浸渍预处理相比，碱预处理2％NaOH明显增强木质素去除，从而将酶水解产率改善至81.2％〜48h。用含有80g / L衬底的批次水解开始喂养批次水解，两次加入纤维素残留物，以得到110g / L的最终底物浓度。水解72小时后，还原糖浓度达到89.5克/升，水解产率为83.3％。使用重组酵母菌菌株酿酒酵母酿酒酵母Zu-10进一步发酵含有56.7g / l葡萄糖和23.6g / L木糖的纤维素水解产物，并在72小时内产生36.3g / l乙醇。从可再生木质纤维素生物质产生的燃料乙醇生产中的研究结果是有意义的

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