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首页> 外文会议>International Conference on Materials for Environmental Protection and Energy Application >Screening of Lipid High Producing Mutant from Rhodotorula Glutinis by Low Ion Implantation and Study on Lipid Extration Technique
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Screening of Lipid High Producing Mutant from Rhodotorula Glutinis by Low Ion Implantation and Study on Lipid Extration Technique

机译：低离子注入与脂质萃取技术的抑制脂质高产突变体的脂质高产突变体

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The paper studied on parameters of ion implantation into lipid producing strain Rhodotorula glutinis and lipid extration technology. It was found that the strain had a higher positive mutation rate when the output power was lOkeV and the dose of N+ implantation was 80x2.6x1013 ions/cm2. Then a high-yield mutant strain D30 was obtained and it's lipid yield which was 3.10 g/L increased by 33.05% than that of the original. Additionally, statistically-based experimental designs were applied for the optimization of lipid extraction by acid-heating coupling ultrasonic technique. By a Plackett-Burman design, it was found that three factors, treatment time of HC1 (p=0.036) , ultrasonic time (p=0.0105) and rate of extracting solution (VCHC13:VCH30H) (p=0.0105), had significant effect on lipid extraction. Subsequently, these significant factors were optimized using response surface methodology (RSM), and the optimized parameters of lipid extraction were as follows: 34 min for treatment time of HC1, 7.5 min for ultrasonic treating time, 1.9:1 for rate of extracting solution (VCHC13:VCH30H). Finally the fermentation characteristic of high-yield mutation strain D30 was studied, when fermentation time was 10 d, it's lipid yield increased to 7.81 g/L
机译：本文研究了离子植入参数，进入脂质生产菌株乳蛋白酶菌株和脂化萃取技术。发现当输出功率是Lokev时，应变具有更高的阳性突变率，并且N +植入剂量为80×2，6×1013离子/ cm 2。然后获得高产率突变菌株D30，其脂质产率为3.10g / L的脂质产率比原始的33.05％增加33.05％。另外，耐酸加热耦合超声技术应用了统计学的实验设计，用于优化脂质提取。通过Plackett-Burman设计，发现三种因素，HC1的治疗时间（P = 0.036），超声波时间（p = 0.0105）和提取溶液（VCHC13：VCH30H）的速率（P = 0.0105），具有显着效果论脂质提取。随后，这些显著因素响应面法（RSM）优化，和脂质提取的优化参数如下：用于HC1的处理时间34分钟，7.5分钟用于超声处理时间，1.9：1，用于提取溶液的速率（ VCHC13：VCH30H）。最后研究了高产突变菌株D30的发酵特性，当发酵时间为10 d时，脂质产量增加到7.81克/升

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