The Expression and Characterization of Gp85 Gene of Subgroup J Avian Leukosis Virus Associated with Hemangioma

机译：血管瘤亚组J禽白血病病毒GP85基因的表达与表征

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The subtype of avian leukosis virus (ALV) was mainly determined by the gp85 glycoprotein. A subtype J ALV strain SCDYl associated with hemangioma was isolated from grandparent breeding chicken and the highly antigenic region of its gp85 gene was amplified and expressed in Rosetta Escherichia coli using the pET-32a(+)vector. The fusion protein, which was expressed at a high level, was similar antigenically to the native gp85 protein as determined by Western blot assay using polyclonal antibodies against ALV-J strain. The fusion protein was also purified. This research lays a foundation for using this recombinant protein for development of indirect enzyme-linked immunosorbent assay (ELISA) for serum antibody detection or for production of monoclonal antibodies against prevalent ALV-J.

机译：禽白血病病毒（ALV）的亚型主要由GP85糖蛋白决定。与血管瘤相关的亚型J Alv菌株苏氏菌氏菌从祖父母繁殖鸡肉中分离，并使用PET-32a（+）载体在罗瑟塔大肠杆菌中扩增并表达其Gp85基因的高抗原区域。在高水位中表达的融合蛋白抗原与天然GP85蛋白相似，通过使用针对Alv-J菌株的多克隆抗体通过Western印迹测定确定。融合蛋白也纯化。该研究为使用这种重组蛋白的基础，用于开发用于血清抗体检测的间接酶联免疫吸附测定（ELISA）或用于对普遍普遍的Alv-J产生单克隆抗体的基础。

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《International Conference on Materials for Environmental Protection and Energy Application》|2012年||共6页
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Min Shi; Yan Lin; Yang Zhao;
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中图分类 TB3-532;
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Subgroup J avuan leukosis virus; gp85 gene; Prokaryotic expression; pET-32a;

机译：亚组J AVUAN白血病病毒;GP85基因;原核表达;宠物32a;

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1. Isolation, identification, and gp85 characterization of a subgroup A avian leukosis virus from a contaminated live Newcastle Disease virus vaccine, first report in China [J] . Zhao P, Dong X, Cui ZZ Poultry Science . 2014,第9期

机译：在中国首次报道的被污染的活新城疫病毒疫苗中甲型禽白血病病毒亚群的分离，鉴定和gp85表征
2. Genetic variability of the Avian leukosis virus subgroup J gp85 gene in layer flocks in Lower Egypt [J] . Nahed Yehia, Hemat S. El-Sayed, Sabry E. Omar, Veterinary World . 2020,第6期

机译：下埃及层群禽白血病病毒亚组J GP85基因的遗传变异
3. Genetic diversity and phylogenetic analysis of glycoprotein gp85 of avian leukosis virus subgroup J wild-bird isolates from Northeast China [J] . JiangL., ZengX., HuaY., Archives of virology . 2014,第7期

机译：中国东北地区禽白血病J亚群野生鸟糖蛋白gp85的遗传多样性和系统发育分析
4. The Expression and Characterization of Gp85 Gene of Subgroup J Avian Leukosis Virus Associated with Hemangioma [C] . Min Shi, Yan Lin, Yang Zhao International Conference on Materials for Environmental Protection and Energy Application . 2012

机译：血管瘤亚组J禽白血病病毒GP85基因的表达与表征
5. Identification and characterization of TNFR-like receptors for subgroups B, D, and E avian leukosis viruses: Two isoforms of a common receptor explain non-reciprocal receptor interference between cytopathic and non-cytopathic avian retroviruses. [D] . Adkins, Heather Breen. 2000

机译：B，D和E组禽类白血病病毒的TNFR样受体的鉴定和特征：共同受体的两种同工型解释了细胞性和非细胞性禽逆转录病毒之间的不可逆受体干扰。
6. Genetic variability of the Avian leukosis virus subgroup J gp85 gene in layer flocks in Lower Egypt [O] . Nahed Yehia, Hemat S. El-Sayed, Sabry E. Omar, 2020

机译：下埃及层群禽白血病病毒亚组J GP85基因的遗传变异
7. Complete Genome Sequence of an American Avian Leukosis Virus Subgroup J Isolate That Causes Hemangiomas and Myeloid Leukosis [O] . Sanandan Malhotra, James Justice, Nathan Lee, 2015

机译：完全基因组序列的美国禽白血病病毒亚组J分离，导致血管瘤和骨髓性白血病

The Expression and Characterization of Gp85 Gene of Subgroup J Avian Leukosis Virus Associated with Hemangioma

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