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Applications of ultrafiltration-nanofiltration membrane continuous combination technology for refining of milk-derived oligosaccharides

机译:超滤 - 纳米过滤膜连续组合技术精制乳衍生寡糖的应用

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The galactooligosaccharide (GOS) was produced by Bulgarian lactic acid bacteria fermentation and further refined by application of an ultrafiltration-nanofiltration continuous membrane combination technology established in this paper. Results revealed that the optimum of membrane operation conditions for ultrafiltration were 0.05 Mpa at the feed concentration of 2.5 g/L, and the optimal conditions for nanofiltration were 45 °C, 0.4 Mpa. After refining by membrane combination technology, 96.70% of proteins were removed and the relative quality ratio of the refined GOS product was increased by 22 times or so, which mainly contained oligomers with DP 3 and DP 2, and mostly distributed between 108 Da and 2415 Da. These findings suggest that the ultrafiltration-nanofiltration continuous membrane combination technology is a new and favorable processes method for enrich or refining of milk-derived GOS obtained by fermentation.
机译:加乳寡糖(GOS)由保加利亚乳酸菌发酵制备,并通过在本文中施加超滤纳米过滤连续膜组合技术进一步改进。结果表明,在2.5g / L的进料浓度下,超滤的膜操作条件的最佳值为0.05MPa,纳米过滤的最佳条件为45℃,0.4MPa。通过膜组合技术精制后,除去96.70%的蛋白质,并将精制的GOS产物的相对质量比增加22次,其主要含有DP 3和DP 2的低聚物,并且主要分布在108DA和2415之间达。这些发现表明,超滤 - 纳米过滤连续膜组合技术是一种新的和有利的方法,用于富集或精炼通过发酵获得的牛奶衍生的GOS。

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