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首页> 外文会议>International Conference on Biomedical Electronics and Devices >LOW-COST ENZYME-BASED BIOSENSOR FOR LACTIC ACID AMPEROMETRIC DETECTION: Electrical Modeling and Validation for Clinical and Food Processing Applications
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LOW-COST ENZYME-BASED BIOSENSOR FOR LACTIC ACID AMPEROMETRIC DETECTION: Electrical Modeling and Validation for Clinical and Food Processing Applications

机译：基于低成本的基于酶的生物传感器，用于乳酸电压检测：临床和食品加工应用的电气建模和验证

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摘要

In this work we present the preliminary resulting measurements of an enzyme-based biosensor for the amperometric detection of lactic acid (LA). The sensor is based on low-cost gold electrodes on polymeric substrate. The redox catalytic enzyme used for analyte amperometric detection is lactate oxidase (LOx) from Pediococcus sp. This enzyme has been immobilized over electrodes surfaces by direct adsorption methodologies. Analysis of the enzyme-modified electrodes have been carried out by means of Electrochemical Impedance Spectroscopy (EIS) and with the development of an equivalent electrical model, in order to improve the adsorption process. Biosensors performance have been evaluated with Cyclic Voltammetry (CVM) measurements in different lactic acid solutions with concentrations from 1 μM up to 300 mM. The lactate sensitivity of this disposable biosensor results in about 6.24 μA mM~(-1) cm~(-2).
机译：在这项工作中，我们介绍了基于酶的生物传感器的初步结果测量，用于乳酸（La）的乳酸（La）的静脉化检测。传感器基于聚合物基材上的低成本金电极。用于分析物慢性检测的氧化还原催化酶是来自Pediococcus SP的乳酸氧化酶（LOX）。通过直接吸附方法，该酶已经通过电极表面固定化。通过电化学阻抗光谱（EIS）和随着等同电气模型的发展，进行了对酶改性电极的分析，以改善吸附过程。生物传感器的性能已经在不同乳酸溶液中的循环伏安法（CVM）测量评估，其浓度从1μm达到300mm。这种一次性生物传感器的乳酸敏感性导致约6.24μAmm〜（-1）cm〜（-2）。

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