• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>International Conference on Applied Mechanics, Materials and Manufacturing >Effects of Bcl-2, bax and p53 Gene Protein Expressions in Gastric Cancer Tissue on the Apoptosis of Cancer Cells
【24h】

Effects of Bcl-2, bax and p53 Gene Protein Expressions in Gastric Cancer Tissue on the Apoptosis of Cancer Cells

机译：Bcl-2，Bax和P53基因蛋白表达在胃癌组织对癌细胞凋亡的影响

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To investigate the expression of p53 gene in gastric cancer tissue and its correlation with bcl-2 and bax protein expression in relationship and discuss the significance of their correlation in clinic. Methods: Pathological specimens from 100 gastric cancer patients with complete medical data and 24 normal lung tissue specimens were selected. Immunohistochemical streptavidin-biotin-peroxidase assay was used to detect the expression of bcl-2, bax and p53 protein. Results: p53 expression was positively correlated with bcl-2 expression (Pearson's R =0.491, P <0.05). The positive expression of p53 was no significantly correlated with bax expression (P> 0.05). The positive rate of p53 expression in the well-differentiated gastric cancer tissues was 17.6%, the positive rate in the differentiated tissues was 90.9%, the positive rate in the poorly differentiated tissues was 72.7%, and There was a significant difference in the expression of p53 in the three tissues (P <0.05). The expression of p53 in the TNM staging showed that the positive rate in stage I and II was 27.3%, the positive rate in stage III and stage IV was 89.3%, the difference between them was significant (P <0.05). Conclusions: The expression of p53 gene has an obvious clinical significance for the assessment of degree of malignancy and the prognosis of gastric cancer. Bcl-2 and p53 gene expression could jointly promote the development of gastric cancer in a synergistic way by acting on different stages of apoptosis, or bcl-2's participating in p53-induced mitochondria-mediated apoptosis signaling pathways. Gastric cancer is one of malignant tumors leading to death today. Tumor formation is a multi-gene, multi-step and multi-stage complex process. In recent years, with the development of research on apoptosis, it has been found that out of control of tumor cell apoptosis and tumor tissue growth are very closely related. Therefore, the study of gastric cancer cell apoptosis has important significance in understanding the development of gastric cancer. In this study, immunohistochemical streptavidinbiotin- peroxidase (SP method) was used to detect the distribution of gene proteins related to apoptosis, such as bcl-2 and bax proteins, in gastric carcinoma; a comparative study of the apoptosis in the tumor tissue was carried out to investigate effects of bcl-2 and bax on the apoptosis of gastric cancer tissue; moreover, the expression of p53, the upstream regulatory gene of bcl-2 and bax in gastric cancer cells, was examined, the correlation of its expression with the expression of bcl-2 and bax was studied, and their interaction mechanism was also explored, which was supposed to provide a theoretical basis for the treatment and prognosis of gastric cancer.
机译：探讨p53基因在胃癌组织中的表达及其与关系中Bcl-2和Bax蛋白表达的相关性，探讨其临床相关性的重要性。方法：选定了100种胃癌患者的病理标本和24例正常肺组织标本。免疫组化链霉抗生物素蛋白 - 生物素 - 过氧化物酶测定法检测Bcl-2，Bax和P53蛋白的表达。结果：P53表达与BCL-2表达呈正相关（Pearson的R = 0.491，P <0.05）。 P53的阳性表达与Bax表达无明显相关（p> 0.05）。良好分化的胃癌组织中P53表达的阳性率为17.6％，分化组织中的阳性率为90.9％，差异化组织中的阳性率为72.7％，表达差异有显着差异P53在三种组织中（P <0.05）。 P53在TNM分期中的表达表明，第I阶段和II阶段的阳性率为27.3％，III期和第四阶段的阳性率为89.3％，它们之间的差异很大（P <0.05）。结论：P53基因的表达对于对恶性肿瘤程度和胃癌预后的评估具有明显的临床意义。 BCL-2和P53基因表达可以通过作用于凋亡的不同阶段，共同促进胃癌的发展，或参与P53诱导的线粒体介导的凋亡信号传导途径。胃癌是今天导致死亡的恶性肿瘤之一。肿瘤形成是多基因，多步骤和多级复杂过程。近年来，随着对凋亡的研究的发展，已经发现失控的肿瘤细胞凋亡和肿瘤组织生长非常密切相关。因此，胃癌细胞凋亡的研究在了解胃癌的发展方面具有重要意义。在该研究中，使用免疫组织化学链霉抗生物素啶生素 - 过氧化物酶（SP方法）检测与胃癌中的细胞凋亡有关的基因蛋白的分布;对肿瘤组织细胞凋亡的比较研究进行了研究Bcl-2和Bax对胃癌组织凋亡的影响;此外，研究了P53，Bcl-2的上游调节基因和胃癌细胞中的上游调节基因的表达，研究了其表达与Bcl-2和Bax表达的相关性，并探讨了它们的相互作用机理，这应该为胃癌的治疗和预后提供理论依据。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号