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首页> 外文会议>SREE Workshop on Medical Materials and Engineering >The effects of high altitude hypoxia environment on ultrastructure and the expression of HIF-1α in the rat lung
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The effects of high altitude hypoxia environment on ultrastructure and the expression of HIF-1α in the rat lung

机译：高海拔缺氧环境对大鼠肺部超微结构及HIF-1α表达的影响

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Background: Studies show that rapid access to high altitude, the body inevitably will be different degrees of injury, more significant damage to heart and lung. The high altitude hypoxia on the lung tissue to adapt to structural changes and hypoxia inducible factor-1α (HIF-1α) expression of the study rarely reported. The experimental area for a short time brought by the near sea level to higher altitudes rat lung tissue by light, electron microscopy and the expression of hypoxia inducible factor-1α changes were studied. Objective: To observe the altitude hypoxia on the ultrastructure of lung tissue and the hypoxia inducible factor-la (HIF-1α) expression. Methods: 50 rats were randomly divided into 5 groups, namely group 1 d Golmud (altitude 2 700 m), 2 d Tanggula group (altitude 5 000 m), 3 d Nagqu group (altitude 4 500 m) and 30 d Nagqu group (altitude 4 500 m), the control group (in Xi'an, elevation 5 m). 4 time-consuming experimental animals 1 d from Xi'an to the Golmud, Qinghai (altitude 2 700 m), 2 d to the Tibetan Tanggula (altitude 5 000 m), 3 d to the Naqu (altitude 4500 m), 30 d in Tibet Nagqu. Light and electron microscopy of the lung tissue samples, Western Blot method to detect the lung tissue of hypoxia inducible factor-1α expression, RT-PCR method for detection of high altitude hypoxic group, lung tissue expression of hypoxia inducible factor-1αmRNA change. Results and Conclusions: Acute hypoxia 2d Tanggula microstructure and ultrastructure of lung tissue apparent high altitude pulmonary edema, and 30d after hypoxic Nagqu group after high altitude pulmonary edema was significantly reduced lung tissue Western blotting, not Canon See HIF-1α protein expression, RT-PCR detection of HIF-1αmRNA in the 30d Nagqu group, a certain amount of expression, the other three experimental groups HIF-1αmRNA expression was not significant (P> 0.05), 30d Nagqu group HIF-lamRNA expression was significantly higher In the control group (P <0.01). The results confirmed that the lung tissue after hypoxic expression of HIF-1αmRNA increase will help reduce the hypoxic altitude pulmonary edema.
机译：背景：研究表明，快速进入高海拔，身体不可避免地会有不同程度的伤害，对心脏和肺部造成重大损害。高海拔缺氧对肺组织适应结构变化和缺氧诱导因子-1α（HIF-1α）的表达很少报道。通过光，电子显微镜和缺氧诱导因子-1α改变的近海拔短时间的实验区以较高的海平面带来更高的大鼠肺组织。目的：观察高度缺氧对肺组织超微结构及缺氧诱导因子--1（HIF-1α）表达的缺氧。方法：将50只大鼠随机分为5组，即第1组D糖（高度2 700米），2d Tanggula组（海拔5 000米），3d Nagqu组（海拔4 500米）和30d Nagqu组（海拔4 500米），对照组（在西安，高度5米）。 4次耗时的实验动物1 d从西安到古代，青海（海拔2 700米），2 d到藏唐格拉（高度5 000米），3 d到Naqu（高度4500米），30 d在西藏纳格斯。肺组织样品的光和电子显微镜，Western印迹法检测缺氧诱导因子-1α表达的肺组织，RT-PCR方法检测高海拔缺氧组，肺组织表达缺氧诱导因子-1αmRNA变化。结果与结论：急性缺氧2D唐格拉微观结构和超微结构肺组织明显高海拔肺水肿，30D高海拔肺水肿后缺氧巨蜥后肺组织Western印迹显着降低，不是佳能见HIF-1α蛋白表达，RT- HIF-1αmRNA的PCR检测在30D Nagqu组中，一定量的表达，其他三个实验组HIF-1αmRNA表达不显着（p> 0.05），对照组的30d Nagqu组HIF-LAMRNA表达显着高得多（P <0.01）。结果证实，HIF-1αmRNA增加缺氧表达后的肺组织有助于减少缺氧高度肺水肿。

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