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首页> 外文会议>SREE Conference on Chemical Engineering >Effects of cadmium pollution in soil on cadmium accumulation of cabbage and its biological effects on human intestinal cells line
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Effects of cadmium pollution in soil on cadmium accumulation of cabbage and its biological effects on human intestinal cells line

机译：镉污染在土壤上对白菜镉积累的影响及其对人肠细胞系的生物学作用

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The pot experiment grown with cabbage was conducted. The initial soil and six incubated soils with different total Cd (0.25, 0.78, 1.3, 3.18, 5.34 and 7.11 mg Cd/kg soil) were used in the pot experiment. The present study was to examine cadmium (Cd) accumulation in edible parts of cabbage and its correlation with Cd concentrations in cabbage garden soil, and evaluate the bioavailability and toxicity of Cd accumulating in cabbage by a human intestinal cell culture model (Caco-2). The results showed that Cd accumulation of cabbage was linearly correlated with Cd concentration in grown soils. Cd uptake of Caco-2 cells increased gradually with increasing level of Cd accumulation in cabbage. MTT assay investigation suggested that viability of the Caco-2 cells reduced with Cd accumulation in cabbage. In comparison with the control group, the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the experiment groups decreased as treated with Cd contaminated cabbages, and there was significant difference in partial groups (p < 0.05). At levels of 47.69 mg Cd kg-1 accumulated in cabbage, the value of T-AOC of cells decreased significantly (p < 0.05), while the content of MDA in cells was significantly higher than that in other groups. The results suggested that Cd concentrations in the edible parts of test cabbage crops were linearly responded to the Cd levels in the growth soil, and toxicological effect of Cd to Caco-2 cells aggravated gradually with Cd accumulation increased in cabbage. .
机译：用卷心菜种植的罐实验。在罐实验中使用初始土壤和六个培养的土壤（0.25,0.78,1.3,3.18,3.18,5.34和7.11mg Cd / kg土壤）。本研究是在白菜庭院土壤中检查白菜可食用部分中的镉（CD）积累及其与CD浓度的相关性，并评估人肠细胞培养模型（Caco-2）中CD积累CD的生物利用度和毒性。结果表明，白菜的CD积聚与生长土壤中的Cd浓度线性相关。 CaC-2细胞的CD吸收随着卷心菜中的CD积累水平逐渐增加。 MTT测定调查表明CaCo-2细胞的活力随卷心菜中的CD积累而降低。与对照组相比，实验组中超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-PX）的活性降低，用CD污染的腹部处理处理，部分群体差异有显着差异（P <0.05）。在卷心菜中累积的47.69mg Cd-1的水平，细胞T-AOC的值显着降低（P <0.05），而细胞中MDA的含量明显高于其他组中的MDA含量。结果表明，测试卷心菜作物的可食用部分中的Cd浓度线性地应对生长土壤中的Cd水平，Cd对Caco-2细胞的毒理学效应逐渐加剧，CD累积在卷心菜中增加。 。

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