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首页> 外文会议>European Conference of the International Federation for Medical and Biological Engineering >Interaction of Human, Canine and Murine Adipose-Derived Stem Cells with Different Biomaterials
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Interaction of Human, Canine and Murine Adipose-Derived Stem Cells with Different Biomaterials

机译：用不同生物材料的人，犬和鼠脂肪衍生干细胞的相互作用

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Due to ease of collection and in vitro expansion, abundance and plasticity, adipose-derived stem cells (ADSC) represent an attractive option for cell therapy and tissue engineering. A variety of biodegradable scaffolds are used as substitutes for the artificial extracellular matrix. Among them are biomaterials consisting of biomolecules such as collagen and poly (L-lactic acid) (OPLA), and bioceramics such as calcium phosphate. As an advantage compared to conventional two-dimensional (2D) cell culture, scaffolds provide an adhesive substrate that also serves as a physical support matrix for cell culture in vitro. The present work aimed to investigate the patterns of adherence and proliferation of human, canine and murine ADSC on these three types of biomaterials. Adipose tissue was obtained from patients undergoing elective liposuction, adult C57BL/6 mice and canine healthy donors. ADSC were isolated with use of collagenase, cultured and characterized. Cells between passages 4 and 7 were associated with 3D scaffolds of calcium phosphate, collagen and OPLA (BD Biosciences), dry or pre-coated with medium with 10% fetal calf serum. Different cell concentrations were used: 104 and 5xl04 cells/scaffold. Cell adherence was evaluated by counting non-adhered cells stained with Giemsa. The proliferation of ADSC in 2D and 3D conditions after 3-day incubation was determined using the MTT test. The cells adhered best to dry scaffolds, and the concentration of 5xl04 cells/scaffold was the most adequate for adherence and proliferation. Human ADSC proliferated more rapidly on 2D than in 3D conditions, contrary to murine and canine cells. These results show that cultivation of ADSC from different species in degradable biomaterials is feasible and may be exploited for the therapeutic use of these compounds.
机译：由于易于收集和体外膨胀，丰度和可塑性，脂肪衍生的干细胞（ADSC）代表细胞疗法和组织工程的吸引力。各种可生物降解的支架用作人造细胞外基质的替代品。其中，是由生物分子组成的生物材料，例如胶原蛋白和聚（L-乳酸）（OPLA）和磷酸钙如磷酸钙等生物陶瓷。作为与常规二维（2D）细胞培养相比的优势，支架提供粘合剂基质，其也用作体外细胞培养物的物理支持基质。目前的作品旨在探讨人，犬和鼠ADSC的粘附和增殖模式在这三种生物材料上。从接受选修吸脂患者，成人C57BL / 6小鼠和犬健康供体获得脂肪组织。使用胶原酶，培养和表征分离ADSC。通道4和7之间的细胞与磷酸钙，胶原醇和OPLA（BD Biosciences）的3D支架相关，用10％胎牛血清的培养基干燥或预涂覆。使用不同的细胞浓度：104和5×10 4个细胞/支架。通过计数用Giemsa染色的未粘附细胞来评估细胞粘附。使用MTT试验测定3天孵育后的2D和3D条件下ADSC的增殖。细胞粘附在干燥的支架上最佳，5×04个细胞/支架的浓度最适合粘附和增殖。人类ADSC在2D中比3D条件更快地增殖，与小鼠和犬细胞相反。这些结果表明，在可降解的生物材料中，来自不同物种的ADSC的培养是可行的，并且可以利用这些化合物的治疗用途。

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