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首页> 外文会议>International conference on germplasm of ornamentals >Analysis of the Germplasm Resources and Genetic Relationships among Cymbidium tortisepalum Cultivars Using Amplified Fragment Length Polymorphism Markers
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Analysis of the Germplasm Resources and Genetic Relationships among Cymbidium tortisepalum Cultivars Using Amplified Fragment Length Polymorphism Markers

机译：用扩增的片段长度多态性标记分析拟康粉底体植物品种的种质资源和遗传关系

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Cymbidium tortisepalum, a Chinese orchid, originated from the basin of Sanjiang, Northwest Yunnan province.This study reports the genetic relationships among 84 cultivars of Cymbidium tortisepalum by amplified fragment length polymorphism (AFLP) markers, including 16 cultivars with plain flowers, 22 cultivars with butterfly-petal-shaped flowers, 19 cultivars with lotus-petal-shaped flowers, 10 cultivars with plum-petal-shaped flowers, and 17 cultivars with poly lips'or poly petals' flowers.Nine AFLP primer combinations produced a total of 1614 fragments with an average of 179 fragments per primer pair, of which 1411 bands were polymorphic.Based on the amplification results, fifty-five of the tested cultivars had a total of 117 specific bands.Unweighted pair group method based on arithmetic average (UPGMA) analysis was performed on dice's similarity coefficient matrix and also average similarity of each cultivar.The dice coefficients among the tested cultivars ranged from 0.1045 to 0.7750, with an average value of 0.4097.The tested 84 cultivars of C.tortisepalum were grouped into four clusters with the similarity coefficient of 0.35.The first cluster consisted of 14 cultivars with plain flowers, 22 cultivars with butterfly-petal-shaped flowers, 15 cultivars with lotus-petal-shaped flowers, and 5 cultivars with plum-petal-shaped flowers.The second cluster included other 2 cultivars with plain flowers.The third cluster contained 17 cultivars with poly lips' or poly petals' flowers, 5 cultivars with plum-petal-shaped flowers, and 3 cultivars with lotus-petal-shaped flowers.The fourth cluster comprised one cultivar with lotus-petal-shaped flowers.These results revealed that the genetic relationships among tested cultivars of C.tortisepalum were related to their flower types' classification.
机译：中国兰花的兰迪姆曲折，来自云南西北部的三江盆地盆地。本研究通过扩增的片段长度多态性（AFLP）标志物报告了84种枸杞曲率曲折的遗传关系，其中包括16种鲜花，22种栽培品种蝴蝶瓣形状的花朵，19个品种莲花叶状花朵，10个品种带梅花瓣形状的花朵，17个品种带有多嘴唇的Poly Petals'Flowers.Nine AFLP引物组合共产生1614个碎片平均每对引物对的179个片段，其中1411条带是多晶族节。基于算术平均值（UPGMA）分析，55个测试品种具有117个特异性的对组方法。对骰子的相似系数矩阵进行，并且每个品种的平均相似性。测试品种的骰子系数为0.1045至0.77 50，平均值为0.4097.将测试的84种C.Tortisepalum含有0.35的相似系数分为四个簇。第一个群组成14种鲜花，22种蝴蝶花瓣状花卉，22种品种。 15种莲花叶状花的栽培品种，5个品种与李子形状的花朵。第二簇包括其他2种带有普通花朵的品种。第三个群集包含17个品种，聚嘴唇或多花瓣'花，5种品种用李子形状的花朵，3种莲花瓣形状的花朵。第四个群体包括一种莲花叶状花朵。结果表明，C.Tortisepalum的测试品种中的遗传关系与之相关他们的花类分类。

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