Transformation of ACC Synthase Gene in Tree Peony, PsACS1, into Nicotiana tabacum

机译：Reg Synthase基因在树牡丹，PSACS1中转化为Nicotiana Tabacum

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Ethylene plays important roles in the regulation of a number of physiological and developmental processes, and is involved in flower opening and senescence of many species.Further researches have been demonstrating that 1-aminocyclopropane-1-carboxylate synthase (ACC synthase, ACS) is a key enzyme regulating the ethylene biosynthetic pathway.To clarify the function of PsACS1 in tree peony, we constructed the sense expression vector of PsACS1 and transferred it into Nicotiana tabacum.Total RNA from the petals of cut tree peony (Paeonia suffruticosa 'Luoyang Hong') was obtained using the CTAB method.The full length eDNA of PsACS1 was cloned by PCR with primers added BamHI and SalI site sequence and transformed into pGM-T vector.After digestion with BamHI and SalI,PsACS1 was ligated with plant expression vector pBI121N in the sense way and the sense plant expression vetor pBIN-35S::PsACS1 was successfully constructed.Moreover, pBIN-35S::PsACS1 was transferred into Agrobacterium tumefaciens GV3101 and then transformed into Nicotiana tabacum 'NC89' through leaf discs.The adventitious shoots and plants were obtained under the selection pressure of 100 mg/L kanamycin.PCR analysis confirmed that PsACS1 was successfully transferred into the genome of Nicotiana tabacum.

机译：乙烯在法规中起重要作用，并参与了许多物种的花开口和衰老。研究已经证明了1-氨基环丙烷-1-羧酸盐合酶（ACC合酶，AC）是a键酶调节乙烯生物合成途径。为了澄清PSACS1在树牡丹中的功能，我们构建了PSACS1的感觉表达载体，并将其转化为烟草树牡丹的花瓣（Paeonia sufforyosa'Luoyang Hong'）使用CTAB方法获得。通过PCR用引物加入BamHI和Sali位点序列的PSACS1全长EDNA，并转化成PGM-T载体。用BamHI和Sali消化，用植物表达载体PBI121n连接Psacs1。感知方式和感觉植物表达vetor pbin-35s :: psacs1已成功构建.Orous，PBIN-35S :: PSACS1转移到Tumefacien中转移到Agrobacterium小号GV3101然后通过叶discs.The不定芽和植物的100毫克的选择压力下获得的转化到烟草“NC89” / L kanamycin.PCR分析证实PsACS1被成功转移至烟草的基因组中。

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来源
《International conference on germplasm of ornamentals》|2013年||共6页
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作者
C.Zhang; Y.H.Zhang; W.N.Wang; P.Y.Jia; L.Dong;
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中图分类观赏园艺（花卉和观赏树木）;
关键词
Paeonia suffruticosa; ethylene; 1-aminocyclopropane-1-carboxylate synthase; sense expression vector; tobacco;

机译：Paeonia suffruticosa;乙烯;1-氨基环丙烷-1-羧酸合酶;感测表达载体;烟草;

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1. Isolation and expression analysis of NtCHS6, a new chalcone synthase gene from Nicotiana tabacum [J] . CHEN Shuai12 ZHANG Yin-chao PAN Xu-hao LI Yi-ting CUI Li-jie WU Feng-yan CAO Mo-ju YANG Ai-guo PAN Guang-tang 农业科学学报：英文版 . 2017,第007期
2. lsolation and expression analysis of NtCHS6, a new chalcone synthase gene from Nicotiana tabacum [J] . CHEN Shuai, ZHANG Yin-chao, PAN Xu-hao, 农业科学学报（英文版） . 2017,第007期
3. Transformation of ACC synthase gene in tree peony, PsACS1, into Nicotiana tabacum. [J] . Zhang C., Zhang Y. H., Wang W. N., Acta Horticulturae . 2013,第977期

机译：将牡丹中的ACC合酶基因PsACS1转化为烟草。
4. Molecular characterization and expression of ethylene biosynthetic genes during cut flower development in tree peony (Paeonia suffruticosa) in response to ethylene and functional analysis of PsACS1 in Arabidopsis thaliana. [J] . Zhou Lin, Zhang Chao, Fu JianXin, Journal of Plant Growth Regulation . 2013,第2期

机译：响应乙烯的牡丹（Paeonia suffruticosa）切花发育过程中乙烯生物合成基因的分子表征和表达以及拟南芥中PsACS1的功能分析。
5. Creation of elite growth and development features in PAP1-programmed red Nicotiana tabacum Xanthi via overexpression of synthetic geranyl pyrophosphate synthase genes [J] . Li Gui, Ji Xiaoming, Xi Jing, Molecular Breeding . 2019,第4期

机译：通过合成的长甲基焦磷酸合成酶基因的过表达，在PAP1编程的红色尼古塔塔纳氏蛋白Xanthi中创建精英增长和开发特征
6. Transformation of ACC Synthase Gene in Tree Peony, PsACS1, into Nicotiana tabacum [C] . C.Zhang, Y.H.Zhang, W.N.Wang, International conference on germplasm of ornamentals . 2013

机译：Reg Synthase基因在树牡丹，PSACS1中转化为Nicotiana Tabacum
7. A reverse genetics approach to investigate the role of CRY1 and CRY2 in mediating floral initiation in the long day plant Nicotiana sylvestris and the short day plant Nicotiana tabacum cv. Maryland mammoth. [D] . Yendrek, Craig R. 2006

机译：一种反向遗传学方法，用于研究CRY1和CRY2在介导长日植物烟草和短日植物烟草cv的花启动中的作用。马里兰猛ma象。
8. Induction of ethylene biosynthesis in Nicotiana tabacum by a Trichoderma viride xylanase is correlated to the accumulation of 1-aminocyclopropane-1-carboxylic acid (ACC) synthase and ACC oxidase transcripts. [O] . A Avni, B A Bailey, A K Mattoo, 1994

机译：木霉木聚糖木聚糖酶诱导烟草中乙烯生物合成与1-氨基环丙烷-1-甲酸（ACC）合酶和ACC氧化酶转录物的积累有关。
9. Induction of ethylene biosynthesis in Nicotiana tabacum by a Trichoderma viride xylanase is correlated to the accumulation of 1-aminocyclopropane-1-carboxylic acid (ACC) synthase and ACC oxidase transcripts. [O] . Avni, A, Bailey, B A, Mattoo, A K, 1994

机译：木霉木聚糖木聚糖酶诱导烟草中乙烯生物合成与1-氨基环丙烷-1-甲酸（ACC）合酶和ACC氧化酶转录物的积累有关。

Transformation of ACC Synthase Gene in Tree Peony, PsACS1, into Nicotiana tabacum

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