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Visualization of Lipid Bodies in Chlamydomonas reinhardtii Living Cells with Fluorescent Microscopy

机译:用荧光显微镜的衣原体Reinhardtii活细胞的脂质体现的可视化

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Acquisition of lipids mainly in a form of triacylglycerol from microalgae is a potential way for biofuels production. Many unicellular microalgae make little triacylglycerol under favorable growth condition, but accumulate large amounts of it under environmental stress conditions (e.g., nutrient depletion, high light). In this paper, the green microalgae Chlamydomonas reinhardtii BAFJ5 was used as a model to study production of lipid bodies (LBs) which are important lipid storage organelles, particularly under stress. We discovered LBs accumulated dramatically when cells were grown in nitrogen-deplete medium. Using fluorescent microscopy and confocal laser scanning microscopy, intracellular LBs can be successfully visualized. The number and size of LBs were also quantified.
机译:从微藻的三酰基甘油的形式采集脂质是生物燃料生产的潜在方法。许多单细胞微藻在良好的生长条件下制造少量三酰基甘油,但在环境压力条件下积累大量的量(例如,营养耗竭,高光)。在本文中,绿色微藻衣原体Reinhardtii Bafj5被用作研究脂质体(LBS)的生产的模型,这是重要的脂质储存细胞器,特别是在应力下。当细胞在氮气 - 耗尽培养基中生长时,我们发现磅积累的LBS。使用荧光显微镜和共聚焦激光扫描显微镜,细胞内LBS可以成功可视化。 LB的数量和大小也被量化。

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