Automated Extraction of Plant Viral RNA Using the Nucleic Acid Instrument Based on Magnetic Nanobeads

机译：基于磁纳米孔的核酸仪器自动提取植物病毒RNA

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In this paper, an automated nucleic acid extraction instrument based on magnetic nanobeads is described. Also, a prototype is designed and constructed. Some plant viral RNA extraction experiments were done using the platform. And the experimental results demonstrated that the instrument could be successfully used for extraction of plant viral RNA, and the extracted RNA was successfully used in an automated PCR assay for the detection of plant virus. The Ct values of CGMMV RNA, LSV RNA and ArMV RNA at the initial concentration were 12.51, 17.81 and 23.48.

机译：本文描述了一种基于磁性纳米形状的自动核酸提取仪。此外，设计和构造了原型。一些植物病毒RNA提取实验使用平台进行。并且实验结果表明，仪器可以成功用于提取植物病毒RNA，并且提取的RNA成功用于自动化PCR测定中以检测植物病毒。 CGMMV RNA，LSV RNA和ARMV RNA在初始浓度下的CT值为12.51,17.81和23.48。

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《International Conference onensors, Measurement and Intelligent Materials》|2013年||共4页
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Yongjie Dou; Xiaoli Zhao; Jiansheng Xu; Congliang Deng; Leng Nie; Yang Yu; Ming Wang; Tao Song;
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原文格式 PDF
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中图分类 TP212-53;
关键词
Magnetic Nanobeads; Nucleic acid extraction; Magnetic bead separation; Plant viral RNA;

机译：磁性纳米珠;核酸提取;磁珠分离;植物病毒RNA;

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1. Advances in Fully Automated Nucleic Acid Extraction System Based on Magnetic Nanobeads [J] . Chen Zhu, Xiao Changhu, Tang Manling, Nanoscience and Nanotechnology Letters . 2019,第12期

机译：基于磁性纳米孔的全自动核酸提取系统进步
2. Research on Automated Nucleic Acid Extraction Instrument Based on Magnetic Nanoparticles Separation [J] . Chen Zhu, Wu Yanqi, Kang Miao, Nanoscience and Nanotechnology Letters . 2018,第1期

机译：基于磁纳米粒子分离的自动核酸提取仪研究
3. Extraction of viral nucleic acids: Comparison of five automated nucleic acid extraction platforms [J] . VerheyenJ., KaiserR., BozicM., Journal of clinical virology: The official publication of the Pan American Society for Clinical Virology . 2012,第3期

机译：病毒核酸的提取：五个自动核酸提取平台的比较
4. Automated Extraction of Plant Viral RNA Using the Nucleic Acid Instrument Based on Magnetic Nanobeads [C] . Yongjie Dou, Xiaoli Zhao, Jiansheng Xu, International Conference onensors, Measurement and Intelligent Materials . 2013

机译：基于磁纳米孔的核酸仪器自动提取植物病毒RNA
5. Magnetic Ionic Liquids in Applications of Nucleic Acid Analysis: Sequence-Specific Extraction of DNA and Preservation Solvents of RNA [D] . Zhu, Chenghui. 2020

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6. Comparison of an automated nucleic acid extraction system with thecolumn-based procedure [O] . Hagen Frickmann, Rebecca Hinz, Ralf Matthias Hagen 2015

机译：自动核酸提取系统与基于列的过程
7. Hardware Integration and Software Design of Automated Nucleic acid extraction System based on Magnetic Bead Separation Method [O] . Yang Yu, Yongjie Dou, Qian Li, 2015

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8. Use of Magnetic Bead Resin and Automated Liquid Handler Extraction Methods to Robotically Isolate Nucleic Acids of Biological Agent Simulates [R] . Seegar, T. C. , Emanuel, P. A. , Bailey, A. M. , 2003

机译：使用磁珠树脂和自动液体处理器提取方法机器人分离生物制剂模拟物的核酸

Automated Extraction of Plant Viral RNA Using the Nucleic Acid Instrument Based on Magnetic Nanobeads

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