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Biofilm forming ability of bacteria isolated from necrotic roots canals of teeth

机译:生物膜形成从坏死根运河分离的细菌的能力

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The growth of microbes in biofilms are associated with repeated and chronic human infections and are extremely resistant to antimicrobial agents. The purpose of this study was to determine the diversity of bacteria from necrotic roots canals of teeth and to detect their biofilm formation ability. A total of 42 bacterial isolates were isolated and identified as belonging to 11 genera. These are Enterococcus sp. (21.4%) followed by Streptococcus sp. (16.8%), Bacillus sp. (11.9%), Peptostreptococcus sp. (9.5%), Staphylococcus sp. (9.5%), Bacteroides sp. (7.1%), Clostridium sp. (7.1%), Actinomyces sp. (7.1%), Fusobacterium sp. (4.76%), Provotella sp. (2.4%) and Chromobacterium sp. (2.4%). Three screening methods for biofilm forming ability were used. Congo Red Agar method (CRA), Tube method (TM) and Microtitre Plate (MTP). From the results, MTP method is a more reliable and quantitative method for the screening and detection of microorganism's ability to form biofilm. This method can be recommended and suggested as a general screening method for the detection of biofilm forming bacteria isolated from roots canals of teeth.
机译:生物膜中微生物的生长与重复和慢性人感染有关,并且对抗微生物剂极其耐药。本研究的目的是确定牙齿坏死根部的细菌的多样性,并检测它们的生物膜形成能力。共分离42个细菌分离物并鉴定为属于11属。这些是肠球菌sp。 (21.4%),然后是链球菌SP。 (16.8%),Bacillus sp。 (11.9%),peptostreptococcus sp。 (9.5%),葡萄球菌SP。 (9.5%),Bacteroides sp。 (7.1%),Clostridium sp。 (7.1%),放大菌菌。 (7.1%),Fusobacterium sp。 (4.76%),Provotella SP。 (2.4%)和Chofobacterium sp。 (2.4%)。使用了用于生物膜形成能力的三种筛选方法。刚果红琼脂法(CRA),管方法(TM)和微量滴定板(MTP)。从结果,MTP方法是一种更可靠和定量的方法,用于筛选和检测微生物形成生物膜的能力。可以推荐这种方法,并建议作为检测从牙齿根部分离的生物膜形成细菌的一般筛选方法。

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