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首页> 外文会议>UKM FST Postgraduate Colloquium >Molecular Identification of Aquilaria spp. by Using Inter-Simple Sequence Repeat (ISSR)
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Molecular Identification of Aquilaria spp. by Using Inter-Simple Sequence Repeat (ISSR)

机译：Aquilaria SPP的分子鉴定。通过使用简单的序列重复（ISSR）

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Aquilaria species are very important economic plant for production of resin locally known as gaharu in Malaysia. There are five species that can be found in Malaysia and the most important Aquilaria species for gaharu production is A. malaccensis. Molecular markers for Aquilaria species are still insufficient and require more efficient, robust and reproducible molecular marker. Inter-simple sequence repeat (ISSR) markers are highly polymorphic and have high reproducibility which will be useful in areas of genetic diversity, phylogenetic studies, gene tagging, genome mapping and evolutionary biology in a wide range of crop species. Five selected ISSR primers were used to identify four Aquilaria species commonly found in Malaysia namely A. malaccensis, A. sub-integra, A. crassna and A. hirta. All the primers showed sufficient polymorphism to distinguish between the four species. Hence, the markers derived from ISSR can be used for molecular identification of Aquilaria spp. in ensuring homogenous species for plantation which may improve the quality of resin derived from known and certified materials.
机译：Aquilaria物种是在马来西亚局部被称为Gaharu的树脂的生产经济植物。在马来西亚可以发现五种物种，最重要的气候属于Gaharu生产的种类是A. malAccensis。 Aquilaria物种的分子标记仍然不足，需要更有效，鲁棒和可重复的分子标记。简单的序列重复（ISSR）标记是高性多态性的，并且具有高再现性，可用于遗传多样性，系统发育研究，基因标记，基因组测绘和进化生物学的各种作物物种。五种选定的ISSR引物用于鉴定常见于马来西亚常见的四种Aquilaria物种，即A. MalAccensis，A. Sub-Integra，A. Crassna和A. Hirta。所有引物显示出足够的多态性以区分四种物种。因此，来自ISSR的标记可用于Aquilaria SPP的分子鉴定。在确保种植园的均质物种中，这可以提高来自已知和认证材料的树脂质量。

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