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首页> 外文会议>International Conference on Material Science and Engineering Technology >Biosurfactant Producing Bacteria with Tensoactive and Emulsifying Properties Isolated from Palm Oil Mill Effluent
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Biosurfactant Producing Bacteria with Tensoactive and Emulsifying Properties Isolated from Palm Oil Mill Effluent

机译：生物表面活性剂，产生棕榈油磨流出物中分离的含有拉焦和乳化性能的细菌

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This study introduces potential biosurfactant producing bacteria found in agro-based wastewater generated by palm oil mills. A total of 38 potential biosurfactant producing bacteria isolated from palm oil mill effluent were qualitatively screened for its tensoactive properties using drop collapse and oil spreading tests. Eleven of the 38 isolates exhibited high surface activity through the drop collapse test. The 11 isolates also exhibited clear zones ranging between diameters 2 to 3 cm through oil spreading test which indicated biosurfactant production. Further evaluations were performed quantitatively for all the 11 isolates in relation to their ability in reducing surface tension and emulsifying capability. Results from Du Nouy ring test demonstrated that the isolates successfully reduced surface tension of water from 72 mNm~(-1) to below 40 mNm~(-1). When tested for emulsifying properties, these isolates displayed high emulsifying activity indicating the presence of biosurfactant production. These 11 isolates namely R06, R07, R08, R10, S08, S09, S10, S11, S12, S15 and S17 were selected and identified using 16S rDNA gene sequence analysis. The results suggested that the isolates belong to the genus Bacillus.
机译：本研究介绍了棕榈油厂产生的农业基废水中发现的潜在的生物活性剂产生细菌。使用下降塌陷和油蔓延试验，总共38种从棕榈油磨流出物中分离的潜在的生物表面活性剂产生的细菌。 38分离物中的11个通过下降折叠试验表现出高表面活性。通过油扩散试验，11分离物也显示出直径2至3cm之间的明显区域，指示生物活性剂的产生。对于其在降低表面张力和乳化能力的能力，定量地对所有11个分离物进行了进一步的评价。 Du Nouy Ring试验结果表明，分离物成功将水的表面张力从72mnm〜（-1）降低至40mnm〜（-1）。当测试乳化性能时，这些分离物显示出高乳化活性，表明存在生物活性剂的存在。这些11分离株即R06，R07，R08，R10，S08，S09，S10，S11，S12，S15和S17使用16S RDNA基因序列分析进行鉴定。结果表明分离株属于芽孢杆菌属。

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