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Extraction of Plant Activator Protein from Botrytis Strain and Activity Detection

机译:从植物体菌株和活性检测中提取植物活化剂蛋白

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In this paper, an activator protein was isolated and identified from crude protein of Botrytis Spp which was separated from the soil of the wheat field with the method of mycelium recycling, breaking and centrifuging, etc. The activator protein appeared a single band on SDS-PAGE whose molecular weight was about 42kD. At the same time, it had a preliminary activity test and investigated on this activator protein. This study indicated that this activator protein is able to promote wheat germination after the deal with it, especially when the density is 3μg/mL, the seed germination is the best. So it is proved that the plant activator protein has really the biological function of improving the wheat germination.
机译:在本文中,分离活化剂蛋白并从肉豆蔻蛋白质的粗蛋白质中鉴定,与麦田的土壤分离,用菌丝菌丝菌丝菌丝液回收,破碎和离心等。活化剂蛋白在SDS上出现单个带 - 分子量约为42kd的页面。同时,它具有初步活性测试并在该活化剂蛋白上研究。本研究表明,该活化剂蛋白能够在处理之后促进小麦萌发,特别是当密度为3μg/ ml时,种子萌发是最好的。因此证明植物活化剂蛋白具有改善小麦萌发的生物学功能。

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