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Bioinspired and Multifunctional Phospholipid Polymer Nanoparticles

机译：Bioinspired和多官能磷脂聚合物纳米粒子

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Photoreactive and cytocompatible polymer nanoparticles for immobilizing and photoinduced releasing proteins were prepared. A water-soluble and amphiphilic phospholipid polymer, poly(2-methacryloyloxyethyl phosphorylcholine (MPC)-co-n-butyl methacrylate (BMA)-co-4-(4-(1-methacryloyloxyethyl)-2-methoxy-5-nitrophenoxy) butyric acid (PL)) (PMB-PL) was synthesized. The PMB-PL underwent a cleavage reaction at the PL unit by photoirradiation at a wavelength of 365 nm. Additionally, the PMB-PL took polymer aggregate in aqueous medium and was used to modify the surface of biodegradable poly(L-lactic acid) (PLA) nanoparticle as an emulsifier. The morphology of the PMB-PL/PLA nanoparticle was spherical and approximately 130 nm in diameter. The carboxylic acid group in the PL unit could be used for immobilization of proteins by covalent bonding. The bound proteins were released by a photoinduced cleavage reaction. Within 60 sec, up to 90% of the immobilized proteins were released by photoirradiation and activity of the protein released in the medium was maintained as well as that the original proteins before immobilization. Octa-arginine (R8) could promote internalization of the protein/PLA/PMB-PL nanoparticles into cells when the R8 was co-immobilized on the nanoparticles. After that, photoirradiation induced protein release from the nanoparticles and proteins distributed more evenly inside cells. From these results, we concluded that PMB-PL/PLA nanoparticles have the potential to be used as smart carriers to deliver proteins to biological systems, such as the inside of living cells.
机译：制备用于固定和光致释放蛋白质的光反应性和细胞相容的聚合物纳米颗粒。水溶性和两亲磷脂聚合物，聚（2-甲基丙烯酰氧基乙基磷胆碱（MPC）-CO-丁基甲基丙烯酸酯（BMA）-CO-4-（4-（1-甲基丙烯酰氧基甲基）-2-甲氧基-5-硝基苯氧基）合成丁酸（PL））（PMB-PL）。 PMB-PL通过波长为365nm的光射，在PL单元处进行切割反应。另外，PMB-PL在水性介质中取聚合物骨料，并用于改变可生物降解的聚（L-乳酸）（PLA）纳米颗粒作为乳化剂的表面。 PMB-PL / PLA纳米粒子的形态是球形的，直径约为130nm。 PL单元中的羧酸基团可用于通过共价键固定蛋白质。通过光致裂解反应释放结合的蛋白质。在60秒内，通过光辐射释放高达90％的固定化蛋白质，并在固定前保持培养基中释放的蛋白质的活性。八藻精氨酸（R8）可以促进蛋白质/ PLA / PMB-PL纳米颗粒的内化进入细胞，当R8共固定在纳米颗粒上时。之后，从纳米颗粒和蛋白质释放的光异性诱导蛋白质释放在细胞内部分布更均匀。从这些结果中，我们得出结论，PMB-PL / PLA纳米颗粒具有用作智能载体的潜力，将蛋白质递送至生物系统，例如活细胞内部。

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