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首页> 外文会议>Meeting of the European Association for Research on Plant Breeding Fodder Crops and Amenity Grasses Section >High Density Array for SNP Genotyping and Mapping in Tetraploid Alfalfa
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High Density Array for SNP Genotyping and Mapping in Tetraploid Alfalfa

机译：用于SNP基因分型和TETRAPLOID ALFALFA中的高密度阵列

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The development of an Illumina iSelect Infinium array that includes approximately 9,277 SNPs identified from sequencing 27 diverse alfalfa (Medicago sativa L.) genotypes can be used to accelerate genome-wide association, mapping and molecular breedingefforts in alfalfa. Individuals from a mapping population obtained from a cross between Altet-4 and NECS-141 segregating for aluminum tolerance, fall regrowth, lignin content and other agronomic characteristics were genotyped using the developed array.The dosage status (nulliplex, simplex, duplex, triplex, or quadruplex) of an individual was successfully distinguished using GenomeStudio software for most of the SNPs evaluated. A total of 3,701 SNPs were polymorphic and segregating in the progeny of the mapping population. The number of SNP with segregation ratios suitable for mapping in tetraploid alfalfa were 1,738 SNPs for simplex x nulliplex, 582 SNPs for duplex x nulliplex, and 426 SNPs for simplex x simplex. TetraploidMap software was used to integrate SNPs from the array with an existing framework map. The colinearity between the resulting linkage map and the Medicago truncatula physical map was evaluated. The resulting high-density linkage map in alfalfa generated from this study can be useful to guide the ongoing assembly of the tetraploid alfalfa genome sequence and to identify genie SNPs in relevant loci associated with key traits of agronomic importance. The iSelect Infinium assay represents a platform for high-throughput SNP genotypingin alfalfa that greatly improves genotyping efficiency and enables detection of allelic dosage in a tetraploid species.
机译：Illumina Iselect Infinium阵列的开发包括约9,277个SNP，其测序27不同的苜蓿（Medicago Sativa L.）基因型可用于加速苜蓿的基因组关联，测绘和分子育种区。从Altet-4和NECS-141之间的交叉中获得的映射群体用于铝耐受，秋季再生，木质素含量和其他农艺特征的个体使用该阵列进行基因分型。剂量状态（Nulliplex，Simplex，双链，三重交流，个人使用GenomeStudio软件为大多数SNPS成功地区分了个体的。总共3,701个SNP是在绘图人群的后代具有多态性和偏析。适用于在四倍体苜蓿中映射的分离比的SNP的数量为1,738个SNP，对于单双工X Nulliplex，582个SNPS，对于单反xX Simplex为426个SNP。 TETRAPLOIDMAP软件用于将SNP与现有框架地图集成在数组中。得到了所得连杆地图和Medicago Truncatula物理图之间的ColineArearity。从该研究产生的苜蓿中产生的高密度连杆地图可用于引导四倍体苜蓿基因组序列的持续组装，并在与农艺重要性的关键特征相关的相关基因座中鉴定Genie SNP。 Iselect Infinium测定代表了高通量SNP Genotypingin苜蓿的平台，大大提高了基因分型效率，并能够检测四倍体物种中的等位基因。

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