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首页> 外文会议>International Conference on Mathematics and Natural Sciences >Isolation and Phylogenetic Characterization of Iron-Sulfur-Oxidizing Heterotrophic Bacteria Indigenous to Nickel Laterite Ores of Sulawesi, Indonesia: Implications for Biohydrometallurgy
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Isolation and Phylogenetic Characterization of Iron-Sulfur-Oxidizing Heterotrophic Bacteria Indigenous to Nickel Laterite Ores of Sulawesi, Indonesia: Implications for Biohydrometallurgy

机译：铁 - 硫 - 氧化异养细菌的分离和系统发育表征亚川核镍镍镍，印度尼西亚：生物氢素冶金的影响

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The main objective of this study was to isolate and phylogenetically identify the indigenous iron-sulfur-oxidizing heterotrophic bacteria capable of bioleaching nickel from laterite mineral ores. The bacteria were isolated from a nickel laterite mine area in South Sulawesi Province, Indonesia. Seven bacterial strains were successfully isolated from laterite mineral ores (strains SKC/S-1 to SKC/S-7) and they were capable of bioleaching of nickel from saprolite and limonite ores. Using EzTaxon-e database, the 16S rRNA gene sequences of the seven bacterial strains were subjected to phylogenetic analysis, resulting in a complete hierarchical classification system, and they were identified as Pseudomonas taiwanensis BCRC 17751 (98.59% similarity), Bacillus subtilis subsp. inaquosorum BGSC 3A28 (99.14% and 99.32% similarities), Paenibacillus pasadenensis SAFN-007 (98.95% and 99.33% similarities), Bacillus methylotrophicus CBMB 205 (99.37% similarity), and Bacillus altitudinis 41KF2b (99.37% similarity). It is noteworthy that members of the phylum Firmicutes (in particular the genus Bacillus) predominated in this study, therefore making them to have the high potential to be candidates for the bioleaching of nickel from laterite mineral ores. To our knowledge, this is the first report on the predominance of the phylum Firmicutes in the Sulawesi laterite mineral ores.
机译：本研究的主要目的是分离和系统发育鉴定能够由红土矿物质生成镍的土着铁硫氧化异养细菌。该细菌从印度尼西亚南苏拉威西省的镍红土矿区分离出来。从红土矿物矿石中成功地分离出七种细菌菌株（菌株SKC / S-1至SKC / S-7），它们能够从皂石和褐铁矿矿石中生成镍。使用eztaxon-e数据库，七种细菌菌株的16s rRNA基因序列进行了系统发育分析，导致完整的分层分类系统，它们被鉴定为Pseudomonas taiwanensis Bcrc 17751（98.59％相似性），枯草芽孢杆菌胚芽。 INAQUOSORUM BGSC 3A28（99.14％和99.32％），Paenibacillus pasadenensis safn-007（98.95％和99.33％相似之处），甲基甲基雌激素CBMB 205（相似性为99.37％），和Bacillus Altitudinis 41KF2B（99.37％相似性）。值得注意的是，在本研究中占主导地位的场地常量（特别是芽孢杆菌）的成员，因此使它们具有高潜力，以获得来自红土矿物质的镍的生物浸出的候选物。据我们所知，这是第一份关于苏拉威矿石矿物矿石中门镇的优势的第一报告。

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