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首页> 外文会议>International Science and Engineering Conference >Study of Antigenic Activity of Experimental Samples of the Conjugated Vaccines Based on Synthetic Oligosaccharide Ligands and CRM197 Carrier Protein against Candida albicans, Aspergillus fumigatus and Pseudomonas aeruginosa
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Study of Antigenic Activity of Experimental Samples of the Conjugated Vaccines Based on Synthetic Oligosaccharide Ligands and CRM197 Carrier Protein against Candida albicans, Aspergillus fumigatus and Pseudomonas aeruginosa

机译：基于合成寡糖配体和CRM197载体对念珠菌念珠菌，Aspergillus fumigatus和假单胞菌铜绿假单胞菌的共轭疫苗实验样品的抗原活性研究

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By now there is no effective treatment for a number of socially significant bacterial and fungal diseases. β-(1→3)-glucans are the principal components of the cell wall of fungi, including Candida albicans, Aspergillus fumigatus and other. At the same time, β-(1→3)-glucans are absent in mammals and man, that makes them promising components of carbohydrate-protein conjugated vaccines for the prevention and treatment of fungal infections. Alginic acid, constructed of β-(l→ 4)-linked mannuronic acid are extracellular polysaccharides produced by Pseudomonas aeruginosa. The protein CRM197 is a non toxic derivative of diphtheria toxin, which is widely used as a safe carrier in conjugated vaccines. The purpose of this study was to investigate the antigenic activity of experimental samples of the conjugated vaccines based on synthetic oligosaccharide ligands and CRM197 carrier protein in the competitive enzyme-linked immunosorbent assay. Two-time immunization Balb/c mice with experimental samples of the conjugated vaccines induced the formation of high titers of specific antibodies. High antibody's avidity to their oligosaccharide ligands was shown in competitive ELISA. These data suggest the relevance of further preclinical trials of the conjugated antifungal vaccine against Candida and Aspergillus and antibacterial vaccine against Pseudomonas.
机译：到现在还没有有效的治疗方法的一些社会显著细菌和真菌疾病。 β-（1→3）葡聚糖是真菌细胞壁的主要部件，包括白色念珠菌，烟曲霉和其它。与此同时，β-（1→3）葡聚糖是哺乳动物和人的不存在，这使得它们有希望用于真菌感染的预防和治疗碳水化合物 - 蛋​​白质缀合疫苗的组分。海藻酸，β-（1→4）的构造 - 连接的甘露糖醛酸是由铜绿假单胞菌产生的胞外多糖。该蛋白质是CRM197白喉毒素，它被广泛地用作在缀合疫苗安全载体的无毒衍生物。本研究的目的是调查基于合成寡糖配体和CRM197载体蛋白在竞争酶联免疫吸附测定法，共轭疫苗的实验样品的抗原活性。两次免疫接种Balb / c小鼠用诱导特异性抗体的高滴度的形成的共轭疫苗的实验样品。高抗体的亲和力，以他们的寡糖配体被显示在竞争ELISA。这些数据表明针对念珠菌和曲霉属和对假单胞菌的抗菌疫苗的缀合的抗真菌疫苗的进一步临床前试验的相关性。

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