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首页> 外文会议>Conference on optical biopsy XIII >Microscopy with UV Surface Excitation (MUSE) for slide-free histology and pathology imaging
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Microscopy with UV Surface Excitation (MUSE) for slide-free histology and pathology imaging

机译：具有紫外线表面激发（Muse）的显微镜，用于无滑动组织学和病理成像

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摘要

A novel microscopy method that takes advantage of shallow photon penetration using ultraviolet-range excitation and exogenous fluorescent stains is described. This approach exploits the intrinsic optical sectioning function when exciting tissue fluorescence from superficial layers to generate images similar to those obtainable from a physically thin-sectioned tissue specimen. UV light in the spectral range from roughly 240-275 nm penetrates only a few microns into the surface of biological specimens, thus eliminating out-of-focus signals that would otherwise arise from deeper tissue layers. Furthermore, UV excitation can be used to simultaneously excite fluorophores emitting across a wide spectral range. The sectioning property of the UV light (as opposed to more conventional illumination in the visible range) removes the need for physical or more elaborate optical sectioning approaches, such as confocal, nonlinear or coherent tomographic methods, to generate acceptable axial resolution. Using a tunable laser, we investigated the effect of excitation wavelength in the 230-350 nm spectral range on excitation depth. The results reveal an optimal wavelength range and suggest that this method can be a fast and reliable approach for rapid imaging of tissue specimens. Some of this range is addressable by currently available and relatively inexpensive LED light sources. MUSE may prove to be a good alternative to conventional, time-consuming, histopathology procedures.
机译：描述了一种使用紫外线激发和外源荧光污渍利用浅光子渗透的新型显微镜方法。当从浅表层激发组织荧光时，该方法利用内在光学切片功能，以产生类似于可从物理薄切片的组织样本的图像的图像。光谱范围内的紫外线从大约240-275nm渗透到生物样本的表面中，从而消除了从更深的组织层出现的焦点信号。此外，UV激发可用于在宽光谱范围内同时激发发射的荧光团。 UV光的截面特性（与可见范围中的更常规照明相反）消除了对物理或更详细的光学切片方法的需要，例如共聚焦，非线性或相干性的断层方法，以产生可接受的轴向分辨率。使用可调谐激光，我们研究了激发波长在激发深度的230-350nm光谱范围内的效果。结果揭示了最佳波长范围，并表明该方法可以是组织标本快速成像的快速可靠的方法。其中一些范围是当前可用的和相对便宜的LED光源可寻址。缪斯可能被证明是常规，耗时，组织病理学程序的良好替代品。

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