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首页> 外文会议>International Conference on Materials Engineering and Information Technology Applications >Research on the Effects of 5-HT1A Receptor in Hippocampal CA1 Region in the Cognition of PTSD Rats
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Research on the Effects of 5-HT1A Receptor in Hippocampal CA1 Region in the Cognition of PTSD Rats

机译：5-HT1A受体在PTSD大鼠认知中5-HT1A受体在海马CA1区域的影响

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Objective: Investigate the expression of 5-HT1A receptor in CA1 area of hippocampus of posttraumatic stress disorder (PTSD) rats and to explore the function of 5-HT1A receptor in the cognitive behavior of PTSD rats. Methods: 72 healthy adult SD rats were randomly divided into 4 groups: A (control group), B (model group), C (control +agonist group) and D (model + agonist group), with 18 rats in each group. The rats in B and C groups were constructed with continuous single stimulation (SPS) to construct the PTSD model rats. 2 days after the model was completed, the CA1 of the hippocampus was located under the Stereotaxic instrument. The rats of B and C groups were injected with 5-HT1A receptor agonist 8-OH-DPAT, A and B group rats were injected with equal amount of normal saline, and the dose was 0.3mg/kg. Morris water maze (MWM) was used to test the learning and memory ability in different groups of rats, The expression of 5-HT1A and 5-HT1A protein in hippocampus was examined by immunohistochemistry and immunofluorescence and Western Blot. Results: It was showed in the MWM experiment that the latency of rats in searching for the underwater platform in group C rats was significantly shorter compared with the group A (P<0.01), B and C group were increased (P<0.01). Compared with group B, group D rats escape the latency was significantly prolonged (P<0.01). The expression of 5-HT1A receptor in CA1 region of hippocampal by immunohistochemistry and immunofluorescence and Western Blot experiments all showed that, Compared with the group A, the other 3 groups were significantly increased (P<0.01), Compared with the group B, the group D was significantly increased (P<0.01), neverthelessly, there was no significant difference between the B group and the C group (P> 0.05). Conclusion: The spatial memory impairment in rats with PTSD maybe associated with the increase of the expression of 5-HT1A receptor in the CA1 region of hippocampus.
机译：目的：探讨5-HT1A受体的创伤后应激障碍（PTSD）大鼠海马CA1区的表达，并探讨在PTSD大鼠的认知行为5-HT1A受体的功能。方法：72只健康成年SD大鼠随机分为4组：A组（对照组），B（模型组），C（对照+激动剂组）和d（模型+激动剂组），18只每组英寸在B，C组的大鼠连续单一刺激（SPS）来构造模型PTSD大鼠构成。完成模型后第2天，海马CA1区位于立体定位仪下。 B，C组的大鼠5-HT1A受体注射激动剂8-OH-DPAT，A和B组大鼠用等量的生理盐水的注入，并且剂量为0.3mg的/公斤。 Morris水迷宫（MWM）中的溶液用于测试大鼠的各组的学习和记忆能力，通过免疫组织化学和免疫荧光和Western印迹检查了海马5-HT1A和5-HT1A蛋白的表达。结果：据显示，在MWM实验，与A组（P <0.01）相比，大鼠在搜索C组大鼠水下平台的潜伏期显著较短，B和C组均升高（P <0.01）。与B组相比，组d大鼠逃避潜伏期显著延长（P <0.01）。 5-HT1A受体的海马的CA1区域中通过免疫组织化学和免疫荧光和Western印迹实验的表达都表明，与A组比较，其他3组均显著增加（P <0.01），与B组相比，组d中显著增加（P <0.01），neverthelessly，又出现了B组和C组（P之间没有差异显著> 0.05）。结论：在大鼠PTSD的空间记忆障碍可能与5-HT1A受体的表达在海马的CA1区域中的增加有关。

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