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Pathological alteration of digestive tissues in blue mussel (Mytillus galloprovincialis) larvae induced by Alexandrium fundyense lysates

机译:Alexandrium Fundyense裂解物诱导的蓝贻贝(Mytilus Galloprovincialis)幼虫消化组织的病理变化

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Bloom of toxic dinoflagellate, Alexandrium fundyense has inflicted significant economic loss of both wild fisheries and aquaculture farming. This toxic dinoflagellate is capable of producing extracellular (exudates) and intracellular toxic compound (lysates), causing mass mortality of shellfish in hatchery operation. Despite its significant impacts, there is lack of study to understand the toxicity mechanism and cellular effect of A. fundyense lysates on shellfish larvae. This study aimed to investigate pathological changes of digestive tissues in blue mussel (Mytilus galloprovincialis) larvae exposed to A. fundyense lysates. Blue mussel larvae were exposed to A. fundyense lysates at a bloom concentration of 1 500 cells·ml~(-1) and sampled for histological assessment at every 0 h, 3 h, 15 h, 24 h, and 48 h after exposure. This study found that pathological changes in digestive tissues occurred as early as 3 h after exposure. Pathological changes included cell vacuolation, necrosis, and cilial exfoliation of style sac. Of these changes, only the prevalence and intensity of cell necrosis increases with increasing exposure duration (from low at 3 h to 15 h to intermediate at 24 h to 48 h). The finding of this study suggests that early detection and appropriate mitigation of A. fundyense bloom is needed to minimize its impact on shellfish hatchery.
机译:Alexandrium Fundyense繁殖的毒性Dinoflagellate造成了野生渔业和水产养殖农业的重大经济损失。这种有毒的Dinoflagelate能够产生细胞外(渗出物)和细胞内有毒化合物(裂解物),导致孵化器操作中贝类的质量死亡率。尽管存在重大影响,但缺乏研究缺乏研究贝类幼虫的毒性机制和细胞效果。本研究旨在探讨蓝贻贝(肉豆蔻血液中)幼虫的消化组织的病理变化,暴露于A. Fundyense Lysates。将蓝贻贝幼虫暴露于A.充满浓度为1 500个细胞·ml·ml·ml·ml·ml〜(-1)的裂解裂解物,并在暴露后每0小时,3 h,15 h,24h和48小时进行取样。本研究发现,暴露后3小时,消化组织的病理变化发生在3小时。病理变化包括细胞真空,坏死和葡萄样式囊的剥离。在这些变化中,只有细胞坏死的患病率和强度随着曝光持续时间的增加而增加(从低于3小时至15小时至24小时至48小时)。本研究的发现表明,需要早期检测和适当减轻A.充满活血绽放,以尽量减少其对贝类孵化场的影响。

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