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首页> 外文会议>International Conference on Biological Science >Histologic observation, identification, and secondary metabolites analysis of endophytic fungi isolated from a medicinal plant, hedychium acuminatum Roscoe
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Histologic observation, identification, and secondary metabolites analysis of endophytic fungi isolated from a medicinal plant, hedychium acuminatum Roscoe

机译：从药用植物中分离的内核真菌的组织学观察，鉴定和次生代谢产物分析，嗜钙菌罗斯科

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Some endophytic fungi live in medicinal plant tissue and could produce some secondary metabolites. This research was done to 1) determine the endophytic fungi position in the Hedychium acuminatum Roscoe. plant tissue by histological observation, 2) identify the endophytic fungi species isolated from the rhizome, leaf sheath, and the leaf of H. acuminatum, and 3) analyze the contents of some secondary metabolites that be produced by the endophytic fungi species isolated from H. acuminatum. The determination of the position of endophytic fungi in the H. acuminatum plant tissue was done by histologic observation. The endophytic fungi were isolated from H. acuminatum plant, then were inoculated on Potato Dextrose Agar medium and incubated at 25 °C during 7 to 14 × 24 h. Each endophytic fungus isolate was identified. Each endophytic fungus isolate was inoculated in Potato Dextrose Broth medium and shook at 120 rpm for 7 × 24 h; then the secondary metabolites content was analyzed using the spectrophotometric method. The research result was: 1) the endophytic fungi mycelium were found on the parenchyma cell wall and intercellular space of rhizome, epidermis and parenchyma cell wall of leaf sheath, epidermis cell wall of leaf; 2) eleven endophytic fungi species were found: Rhizoctonia sp., Fusarium solani, F. oxysporum, Colletotrichum alienum, C. aotearoa, C. ti, Aspergillus parasiticus, C. coccodes, C. gloeosporoides, Hansfordia biophila, and F. semitectum; 3) the secondary metabolites content in fungi culture in broth medium are: alkaloid 20.48 μm·mL~(-1) to 117.12 μm·mL~(-1)), flavonoid (148.48 μm·mL~(-1) to 816.96 μm·mL~(-1)), terpenoid (4.73 μm·mL~(-1) to 32.98 μm·mL~(-1)), and tannin (9.39 μm·mL~(-1) to 53.02 μm·mL~(-1)).
机译：一些内生真菌生活在药用植物组织中，可以产生一些次级代谢物。该研究已经完成为1）确定Hedychium acuminatum roscoe中的内生真菌位置。通过组织学观察植物组织，2）鉴定从根茎，叶鞘和H. aciminatum的叶片中分离的内生真菌物种，以及3）分析由H中分离的内生真菌物种产生的一些次生代谢物的含量。扫螨。通过组织学观察来确定H.螨虫植物组织中内生真菌的位置。从H.拟核植物中分离内生真菌，然后接种在马铃薯右旋糖琼脂介质上，并在7至14×24小时内在25℃下温育。鉴定了每个内心真菌菌株。将每个内胚性真菌分离物接种在马铃薯右旋糖浆培养基中，并以120rpm摇晃7×24小时;然后使用分光光度法分析次级代谢物含量。研究结果是：1）内生真菌菌丝体在薄壁蛋白细胞壁和叶片，表皮细胞壁的叶子鞘，表皮和实质细胞壁中发现。 2）发现11种内心真菌物种：Rhizoctonia Sp。，Fusarium solani，F. Oxysporum，Colletotrichum Alienum，C.Aotearoa，C.TI，曲霉，C.Coccodes，C.GroooSporoides，Hansfordia Biophila和F.晶体; 3）肉汤介质中真菌培养中的次级代谢产物含量为：生物碱20.48μm·ml〜（-1）至117.12μm·mL〜（-1）），黄酮（148.48μm·ml〜（-1）至816.96μm ·ml〜（-1）），萜类化合物（4.73μm·ml〜（-1）至32.98μm·ml〜（-1）），单宁（9.39μm·ml〜（-1）至53.02μm·ml〜 （-1））。

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