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Effects of humic acid on vegetative growth, yield, oxalic acid and betacyanin content of red amaranth (Amaranthus tricolor L.)

机译:腐殖酸对红苋(Amaranthus Tricolor L.)营养生长,产率,草酸和贝辛胺碱含量的影响

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Humic acid is biostimulan that has a negative charge and it is able to bind cations that are important in the process of plant growth. Red amaranth (Amaranthus tricolor L.) is one of vegetables that contains betacyanin which is a good source of antioxidants. However, the stem and leaves of red amaranth also contains oxalate which could inhibits calcium absorption in human's body. This study was aimed to evaluated the effect of humic acid on vegetative growth, yield, oxalic acid and betacyanin content of red amaranth (Amaranthus tricolor L.). Red amaranth seeds were germinated in a polybag containing top soil as growth media. Seedling were selected and humic acid were applied through foliage spray or soil surface when plants were at 2 weeks old. The concentration variation of humic acid were 0 mg/L, 5 mg/L, 20 mg/L or 35 mg/L and there were 5 replicates for each treatment. Vegetative growth observed were plant height and leaf number, yield observed were fresh and dry weight of shoot and root. Oxalic acid content was determined by permanganometric titration method, and the density of Ca-oxalate crystal was observed from stem cut preparate made by free-hand section technique. Betacyanin content was determined by HPLC (High Performance Liquid Chromatography), and chlorophyll content was determined by spectrophometric method. Data were analyzed by One Way ANOVA (Analysis of Variance), followed by Duncan Multiple Range Test with 95% confidence level. Results showed that humic acid of 20 mg/L applied through the leaves significantly increase plant height, leaf number, the fresh and dry weight of shoot and betacyanin content. Humic acid of 20 mg/L that was applied through the soil surface also significantly increased leaf number, both fresh and dry weight of shoot and root but reduced oxalate content. From this study it can be concluded that humic acid of 20 mg/L applied either through foliage spay or soil surface increased plant height, yield and betacyanin content but it reduce doxalate content of red amaranth.
机译:腐殖酸是具有负电荷的生物硫醇,它能够结合在植物生长过程中重要的阳离子。 Red Amaranth(Amaranthus Tricolor L.)是含有Betacyanin的蔬菜之一,这是一种良好的抗氧化剂来源。然而,红色苋菜的茎和叶也含有草酸盐,这可能抑制人体中的钙吸收。本研究旨在评估腐殖酸对红色苋菜(Amaranthus Tricolor L)的营养生长,产率,草酸和贝辛胺含量的影响。 Red Amaranth种子在含有顶部土壤中作为生长介质的含有顶部土方制的萌芽。选择幼苗,当植物在2周龄时,通过叶子喷雾或土壤表面施加蜂蜜酸。腐殖酸的浓度变化为0mg / L,5mg / L,20mg / L或35mg / L,每种处理有5种重复。观察到的植物生长是植物高度和叶片数,观察到的产量是新鲜和干重的芽和根。通过高锰酸滴定法测定草酸含量,并从通过自由剖面技术从茎切割制备的茎切割制备的Ca-草酸酯晶体的密度。贝辛烷蛋白含量通过HPLC(高效液相色谱)测定,通过分光尺寸法测定叶绿素含量。通过单向ANOVA(方差分析)进行分析数据,其次是Duncan多范围测试,置信水平95%。结果表明,通过叶片施加20mg / L的腐殖酸显着增加了植物高度,叶子数,芽的芽和甜菜碱含量。通过土壤表面施加20毫克/升的腐殖酸也显着增加了叶子数,芽和根部的新鲜和干重,但也减少了草酸盐含量。从本研究中可以得出结论,通过叶子喷洒或土壤表面增加植物高度,产量和贝辛胺含量的植物酸20mg / l施用,但它降低了红苋菜的道甲酸盐含量。

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