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首页> 外文会议>International Conference on Biological Science >Genetic variation analysis of melon (Cucumis melo L. 'Tacapa Gold') using inter-simple sequence repeat
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Genetic variation analysis of melon (Cucumis melo L. 'Tacapa Gold') using inter-simple sequence repeat

机译：使用简单序列重复的瓜瓜（Cucumis Melo L.'TaCaPa Gold'）的遗传变异分析

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Melon (Cucumis melo L.) is one of the most favorite fruit in Indonesia because of its high contains of nutrients. Melon 'Tacapa Gold' is produced by natural polination between melon 'Tacapa Silver' and 'Hikapel'. The phenotype of 'Tacapa Gold' cultivar has been identified, but its genetic variation has not been studied yet. This study aimed to analyze the genetic variation of melon cultivar 'Tacapa Gold' compared to 'Tacapa Silver', 'Hikapel', and two commercial melons ('Aramis' and 'PI 371795'). PCR-ISSR method with four random primers was used to evaluate genetic variation in melon. The level of genetic similarity was determined by simple matching coefficient (SSM) and the dendrogram was constructed by clustering analysis with unweighted pair-group using arithmetic averages (UPGMA). The research was started with melon seeds germination and cultivation, samples preparation, DNA extraction, DNA qualitative test, DNA quantitative test, DNA amplification using PCR-ISSR, amplification analyzing with electrophoresis, and molecular data analyzing. The results of scoring test were analyzed with MVSP 3.1A program. Four random primers (UBC-808, UBC-809, UBC-811, UBC-824) yielded 26 monomorphic DNA bands and 25 polymorphic DNA bands. Similarity indexes of cultivar melon 'Tacapa Gold' with 'Tacapa Silver', 'Hikapel', 'PI 371795', and 'Aramis' melons were 76.7%; 71.4%; 58.3%; 65.2% respectively. It could be concluded that melon 'Tacapa Gold' had the closest relationship with 'Tacapa Silver' and the farthest was melon 'PI 371795'.
机译：甜瓜（Cucumis Melo L.）是印度尼西亚最喜欢的水果之一，因为它的营养素高。甜瓜'塔帕帕金'是由甜瓜'塔帕帕银'和'hikapel'之间的自然润润。已经确定了“塔帕帕金”品种的表型，但尚未研究其遗传变异。该研究旨在分析甜瓜品种“塔帕帕金”的遗传变异与“塔帕饼银”，“Hikapel”和两个商用瓜（'Aramis'和'Pi 371795'）。具有四种随机引物的PCR-ISSR方法用于评估甜瓜的遗传变异。通过简单的匹配系数（SSM）确定遗传相似度，并且通过使用算术平均值（UPGMA）与未加权对组进行聚类分析来构建树状图。该研究开始于甜瓜种子萌发和培养，样品制备，DNA提取，DNA定性试验，DNA定量测试，使用PCR-ISSR进行DNA扩增，用电泳分析和分子数据分析。使用MVSP 3.1A程序分析评分试验结果。四种随机引物（UBC-808，UBC-809，UBC-811，UBC-824）产生26个单声道DNA带和25个多态性DNA带。 “TaCaPa Silver”，'Hikapel'，'Pi 371795'和'Aramis'瓜的品种甜瓜'TaCaP Gold'的相似性指数为76.7％; 71.4％; 58.3％;分别为65.2％。可以得出结论，甜瓜'塔帕帕金'与“塔帕帕银”的关系最接近，最远的是甜瓜'PI 371795'。

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