• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>SPE/IADC Middle East Drilling Technology Conference and Exhibition >ATP Bioluminescence Assay for Onsite Microbial Assessment in Drilling Rig Operations
【24h】

ATP Bioluminescence Assay for Onsite Microbial Assessment in Drilling Rig Operations

机译：钻机运营中的现场微生物评估ATP生物发光测定

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Deep drilling often requires the use of circulating fluids to facilitate the drilling process, carrying cuttings and rock fragments to the surface. In this practice, microbial contamination and extensive growth of bacteria in the drilling fluids impact drilling operation and reservoir integrity. An onsite and rapid microbial assessment technique is required to provide an early warning of microbial activities in drilling fluids, and manage microbial risks through proper countermeasures. Bioluminescence method is a rapid testing technique for quantification of living cells based on the measurement of adenosine-5'-triphosphate (ATP) found in the sample. ATP test has been widely used in food and pharmaceutical industries; however, its application in the oil and gas industry has been hindered by many interference substances present in typical oil industry samples. In this study, we have validated an ATP-based bioluminescence test for its linearity and repeatability in various types of samples from drilling rig operations (make-up water, drilling mud, and cement mix fluid). The results from ATP test showed an excellent linear relationship with the microbial numbers in the samples determined by plate count method. The study proved ATP bioluminescence technique as a reliable method for onsite determination of microbial contamination in drilling operations, obtaining results in minutes, rather than days or weeks with traditional methods. The action levels based on ATP measurements were recommended for drilling engineers to assess microbial risks in drilling fluids. The implementation of this onsite microbial monitoring technique in the field will facilitate real-time detection and immediate mitigation countermeasures for microbial control in drilling fluids, reducing risks such as loss of rheological properties of drilling fluids from biodegradation, H2S production during drilling downtime and deep in the formation, biomass plugging in the formation, and microbial corrosion. This is the first study of the use of ATP-based test to assess microbial contamination in drilling rig operations.
机译：深钻通常需要使用循环流体来促进钻井过程，将切割和岩石碎片携带到表面。在这种实践中，微生物污染和细菌的广泛生长钻井液冲击钻井操作和储层完整性。需要现场和快速的微生物评估技术来提供钻井液中微生物活性的预警，并通过适当的对策管理微生物风险。生物发光方法是基于样品中发现的腺苷-5'-三磷酸（ATP）的测量来定量活细胞的快速测试技术。 ATP测试已广泛用于食品和制药行业;然而，它在石油和天然气行业中的应用已经受到典型石油工业样品中存在的许多干涉物质。在这项研究中，我们已经验证了基于ATP的生物发光测试，以便在钻机操作（化妆水，钻井泥浆和水泥混合物）中的各种样品中的线性度和可重复性。 ATP测试的结果显示出通过板数法测定的样品中的微生物数的优异的线性关系。该研究证明ATP生物发光技术作为现场测定钻井作业中微生物污染的可靠方法，从分钟内获得结果，而不是传统方法的几天或数周。建议钻探工程师以评估钻井液中的微生物风险，建议基于ATP测量的动作水平。该领域现场微生物监测技术的实施将促进用于钻井液中微生物控制的实时检测和立即缓解对策，降低钻井液中钻井液的流变性能损失，H2S在钻井停机时间和深处形成，生物质堵塞形成，以及微生物腐蚀。这是第一次研究采用ATP的测试，以评估钻机操作中的微生物污染。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号