• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>International Conference on Biological Sciences and Technology >Immunoassays of Methionine Adenosyltransferase Activity, S-adenosylmethione and Their Applications
【24h】

Immunoassays of Methionine Adenosyltransferase Activity, S-adenosylmethione and Their Applications

机译：甲硫氨酸亚苯基转移酶活性，S-腺苷聚氨基及其应用的免疫测定

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To understand how S-adenosylmethione (SAM) fluctuates in normal and cancerous liver cells, we developed novel methods for measuring methionine adenosyltransferase (MAT) activity and SAM. This method integrated MAT-catalyzed reaction and immunoassay that specifically and quantifies product SAM. IHC, IF and ELISA were used in measuring SAM and S-adenosylhomocysteine (SAH). SAM is mostly found in cytoplasm and less in nucleus whereas SAH is mostly found in nucleus. Levels of SAM and SAH were found to be reduced in cancer cells except for early stage when inflammation is boosted to fight against cancerous or other cell damages. 0.5mM methionine stimulated while S-nitrosoglutathione inhibited MAT-I/III activity and methylation index increased after methionine stimulationof L02 cells. Methionine inhibited while S-nitrosoglutathione had no effects on MAT-II activity and methylation index decreased after methionine stimulationof HepG2 cells. Immunoassays described provide better venues for further research on measuring MAT, SAM and SAH under various scenarios.
机译：为了了解如何在正常和癌性肝细胞中波动的S-腺苷（SAM）波动，我们开发了测量蛋氨酸腺苷转移酶（MAT）活性和SAM的新方法。该方法专门地集成了垫催化的反应和免疫测量，并定量了产品SAM。 IHC，IF和ELISA用于测量SAM和S-腺眼囊肌细胞（SAH）。 SAM大多在细胞质中发现，少量核心，而Sah大多是在核中发现的。发现SAM和SAH的水平在癌细胞中被降低，除了早期炎症造成炎症以抵抗癌症或其他细胞损害。 0.5mm蛋氨酸刺激，而S-硝基葡萄抑制率抑制MAT-I / III活性和甲硫氨酸刺激后的甲硫氨酸刺激后的甲硫氨酸的活性和甲基化指数增加。甲硫氨酸抑制，而S-Nitrosogluthathione对Mat-II活性的影响和甲硫氨酸刺激后的甲硫氨酸刺激后的甲基化指数降低。 ImmunoAssays在各种情况下提供了进一步研究测量垫，SAM和SAH的进一步研究。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号