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首页> 外文会议>PRiME Joint International Meeting of the Electrochemical Society, the Electrochemical Society of Japan, and the Korean Electrochemical Society >Surface plasmon resonance (SPR) sensing based on DNA elongation by site-selective surface plasmon (SP) field heating toward ultra-high sensitive detection of single pathogenic particles
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Surface plasmon resonance (SPR) sensing based on DNA elongation by site-selective surface plasmon (SP) field heating toward ultra-high sensitive detection of single pathogenic particles

机译：基于DNA伸长的位点选择性表面等离子体（SP）场加热朝向单一致病颗粒的超高敏感检测，表面等离子体共振（SPR）感测

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In this study, site-selective enzymatic DNA elongation from gold surface at 25°C was induced by the damping heat of propagating surface plasmon (SP), using an 808 nm laser. A multispot immunoassay, based on this concept, has been demonstrated. The primer-immobilized analyte-bound ligand spot was selectively heated by the SP field, and effective DNA elongation on the surface using rolling circle amplification produced an intense fluorescence spot on the surface after staining. The key feature of this method is the temperature contrast on the surface. We have estimated the surface temperature by comparison of the yield and chain length of the amplicons from SP heating to those from controlled thermal reactions at a series of temperatures.
机译：在该研究中，使用808nm激光通过展开表面等离子体（SP）的阻尼热，诱导来自25℃的金表面的位点选择性酶DNA伸长。已经证明了基于该概念的多点免疫测定。通过SP场选择性地加热引物固定化分析物结合的配体点，并且使用滚动圆扩增在表面上有效的DNA伸长在染色后在表面上产生强烈的荧光点。该方法的关键特征是表面上的温度对比度。通过将扩增子的产量和链长从Sp加热与来自一系列温度的受控热反应的那些进行比较，我们估计了表面温度。

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