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首页> 外文会议>Annual^Meeting of the National^Mastitis^Council >Identification and Characterization of Virulence Factors of Staphylococcus aureus Isolates from Cases of Bovine Mastitis
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Identification and Characterization of Virulence Factors of Staphylococcus aureus Isolates from Cases of Bovine Mastitis

机译：牛瘤乳腺炎病原体分离株毒力因子的鉴定与表征

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Bovine mastitis caused by Staphylococcus aureus is a major economically important disease affecting the dairy industry worldwide (Hortet and Seegers, 1998). Currently available vaccines aimed at enhancing intramammary immunity of dairy cows against S.aureus mastitis have had limited success. Major problems affecting the successful development of protective vaccine against S. aureus mastitis are strain variation, presence of exopolysaccharide layer, which enables bacterium to evade host immune system, dilution of immune effectors by large volume of milk (Yancey, 1999), interactions between milk components and immune effectors (Russell et al., 1977) that reduce their effectiveness, and the ability of most mastitis-causing bacteria to attach and internalize into mammary epithelial cells. Staphylococcus aureus attaches to mammary epithelial cells (Hensen et al., 2000) and subsequently form biofilm (Melchior et al., 2006) or may become electron-transport-defective slow-growing small colony variant (Ahmed, 2015, Mirani et al., 2015). It is important to identify and target the most critical factors determining S. aureus colonization of the bovine mammary glands. The overall aim of this project was to identify critical virulence factors of S. aureus expressed during early host-cell-bacterial interactions. Fifty eight S. aureus strains from bovine mastitis were tested by PCR for 16 known virulence factors and for production of biofilm on Congo red agar. All strains except one produce biofilm and most ofthem carry major virulence genes.
机译：由金黄色葡萄球菌引起的牛乳腺炎是影响全球乳制品（Hortet和Seegers，1998）的主要经济上重要的疾病。目前可用的疫苗旨在提高乳制品奶牛的Intramichary免疫性针对S.aureus乳腺炎的成功有限。影响保护疫苗成功发展对金黄色葡萄球菌乳腺炎的主要问题是菌株变异，存在的潜水层，使细菌能够逃避宿主免疫系统，通过大量的牛奶稀释免疫效应器（yancy，1999），牛奶之间的相互作用组分和免疫效应器（Russell等，1977），减少其有效性，以及大多数乳腺炎的能力，使细菌附着和内化入乳腺上皮细胞。金黄色葡萄球菌附着于乳腺上皮细胞（Hensen等，2000），随后形成生物膜（Melchior等，2006），或者可能成为电子运输缺陷的小菌落变体（Ahmed，2015，Mirani等人。 ，2015）。重要的是要识别和瞄准决定牛乳腺殖民群的最关键因素。该项目的总体目标是识别在早期宿主细胞细菌相互作用期间表达的金黄色葡萄球菌的临界毒力因子。从牛乳腺炎的五十八个金黄色葡萄球菌的菌株通过PCR进行PCR进行16名已知的毒力因子，并在刚果红琼脂上生产生物膜。除了一个外膜外，所有菌株都会产生生物膜，大多数血液携带主要的毒力基因。

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