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首页> 外文会议>Conference on biomedical vibrational spectroscopy: Advances in research and industry >Combination of micro-dialysis and infrared spectroscopy: a multi-analyte assay for accurate biofluid analysis and patient monitoring
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Combination of micro-dialysis and infrared spectroscopy: a multi-analyte assay for accurate biofluid analysis and patient monitoring

机译：微透析和红外光谱的组合：用于精确生物流体分析和患者监测的多分析物测定

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摘要

Micro-dialysis can be used for continuously harvesting body fluids, while a multi-component analysis of the dialysates by infrared spectrometry offers splendid opportunities for monitoring substrates and metabolites such as glucose, lactate and others small enough to penetrate the semi-permeable dialysis membranes. However, a drawback of this process are variable recovery rates, which can be observed especially for subcutaneously implanted catheters in human subjects. Isotonic perfusates were investigated with acetate and mannitol as recovery markers for the dialysis of human serum at 37°C to mimic in vivo patient monitoring. The latter non-ionic substance has been suggested for application when other ionic substances such as bicarbonate or pH are also to be determined. Simultaneously for acetate and mannitol, the depletion of the marker substances from the perfusates using different micro-dialysis devices was investigated under various flow-rates. Relationships between relative dialysate marker concentrations and glucose recovery rates were determined based on multivariate calibrations. For quantification, classical least squares with reference spectra for modelling the serum dialysates was used, rendering a basis for reliable blood glucose and lactate measurements.
机译：微透析可用于连续收获体液，而红外光谱法的透析酶的多组分分析提供了用于监测底物和代谢物，例如葡萄糖，乳酸和其他足够小的机会，以渗透半透透析膜。然而，该过程的缺点是可变的回收率，其可以特别是对于在人受试者中皮下植入的导管。在37℃下用乙酸盐和甘露甘露醇作为透析标志物的乙酸盐和甘露醇作为恢复标志物进行研究，以模仿体内患者监测。当也确定其他离子物质如诸如碳酸氢盐或pH的离子物质时，已经提出了后一种非离子物质。对于乙酸盐和甘露醇同时，在各种流速下研究了使用不同微透析器件的灌注液中标记物质的耗尽。基于多变量校准测定相对透析酸盐标志物浓度和葡萄糖回收率之间的关系。为了定量，使用具有用于建模血清透析酸盐的参考光谱的经典最小二乘，为可靠的血糖和乳酸测量进行依据。

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