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首页> 外文会议>International Biology Conference >Soil Burial Method for Plastic Degradation Performed By Pseudomonas PL-01, Bacillus PL-01, and Indigenous Bacteria
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Soil Burial Method for Plastic Degradation Performed By Pseudomonas PL-01, Bacillus PL-01, and Indigenous Bacteria

机译：Pseudomonas pl-01，Bacillus pl-01和土着细菌进行塑性降解土壤埋地方法

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Lately, plastic bag is becoming the most important pollutant for environment since it is difficult to be naturally degraded due to it consists of long hydrocarbon polymer chains. Our previous study indicated that our pure isolate Pseudomonas PL-01 and Bacillus PL-01 could degrade about 10% plastic bag. This present study was aimed to find out whether Pseudomonas PL01 and Bacillus PL01 put a positive effect to indigenous bacteria from marginal area in doing plastic degradation with a soil burial method. Beach sand was used as a representative marginal area, and mangrove sediment was used as a comparison. Plastics were submerged into unsterile beach sand with 10% of Pseudomonas PL-01 or Bacillus PL-01 containing liquid minimal salt medium (MSM) separately, while other plastics were submerged into unsterile mangrove sediments. After 4, 8, 12 and 16 weeks, their biofilm formation on their plastic surfaces and plastic degradation were measured. Results indicated that those 2 isolates put positive influent on biofilm formation and plastic degradation for indigenous beach sand bacteria. Bacillus PL-01 put higher influent than Pseudomonas PL-01. Plastic transparent was preferable degraded than black and white plastic bag 'kresek'. But anyhow, indigenous mangrove soil bacteria showed the best performance in biofilm formation and plastic degradation, even without Pseudomonas PL-01 or Bacillus PL-01 addition. Fourier Transform Infrared (FTIR) analysis complemented the results; there were attenuated peaks with decreasing peaks transmittances. This FTIR peaks indicated chemical functional group changes happened among the plastic compounds after 16 weeks incubation time.
机译：最近，塑料袋成为环境中最重要的污染物，因为由于它由长的碳氢化合物聚合物链组成，因此难以自然地降级。我们以前的研究表明，我们的纯分离物假单胞菌PL-01和Bacillus PL-01可以降低约10％的塑料袋。本研究旨在了解Pseudomonas pl01和芽孢杆菌pl01是否对来自边缘区域的土着细菌对塑性降解的土着细菌进行积极影响。海滩砂被用作代表性边缘地区，红树林沉积物用作比较。塑料被浸没到未消毒的海滩沙假单胞菌PL-01或芽孢杆菌PL-01含有液体基本盐培养基（MSM）分开，而其他塑料被浸没到未消毒的红树沉积物的10％。在4,8,12和16周后，测量它们的塑料表面和塑料降解的生物膜形成。结果表明，这2个分离物对土着海滩砂细菌的生物膜形成和塑性降解对阳性流入。 Bacillus pl-01将较高的流入Pssudomonas pl-01置于较高的影响。塑料透明比黑白塑料袋'Kresek更优选降解。但无论如何，土着红树林土壤细菌表明，生物膜形成和塑料降解的最佳性能，即使没有假鼠PL-01或Bacillus pl-01添加。傅里叶变换红外（FTIR）分析补充了结果;随着峰值透射率降低的衰减峰。该FTIR峰表示塑料化合物在孵育时间16周后发生的化学官能团变化。

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