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Activation of inoculum microorganism from dairy cattle feces

机译：奶牛粪便中的接种微生物的激活

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Coal produces Coal Bed Methane (CBM) which is formed both biogenically and thermogenically. Lignite is not utilized optimally because it has low heat content and productivity time limit that decreases CBM production. In order to utilize lignite waste, adding inoculum consortium microorganism from dairy cattle waste as starter for biogas process can be a solution. This study aimed to produce inoculum consortium microorganism as biogas starter from dairy cattle feces through in vitro activation process by Theoudorou modification method. The research used complete randomized design with 3 replications. The treatments were blank (R_0), 100% concentrate (R_1), 70% concentrate+30% grass (R_2), 70% grass+30% concentrate (R_3) and 100% grass (R_4). All treatments were added by buffer solution and feces with ratio of 2:1 into 100 ml serum injection bottle with anaerobic conditions. The parameters observed were gas production, pH and gas kinetics (orskov's equation) for 2, 4, 6, 8, 10, 12, 24 and 48 hours. The results showed that the treatment had significant effect (P <0.05) on the observed parameters. The highest total gas production was for R_2 and R_3 treatments with total production of 91.17 ml and 101.17 ml, pH (6.62 and 6.57), maximum gas production (94.03 and 97.62 ml), speed of gas production (0.066 and 0.084 ml/hour). There is not a significant difference for both the treatments. The source of inoculum consortium microorganisms for biogas starter selected based on the observed parameters and potential availability of proteolytic and fibrocytic microorganisms is R_2 (70% concentrate +30% grass).
机译：煤炭生产煤层（CBM），其在生物和热化上形成。褐煤未最佳地使用，因为它具有低热含量和降低CBM生产的生产率时间限制。为了利用褐煤废弃物，添加来自乳制品粪便浪费的接种物质微生物作为沼气过程的起动器可以是一种解决方案。本研究旨在通过欧德鲁修改方法通过体外激活过程生产因奶牛粪便的沼气发射器的接种物质微生物。该研究使用完整的随机设计，具有3个复制。该处理是坯料（R_0），100％浓缩物（R_1），70％浓缩+ 30％草（R_2），70％草+ 30％浓缩物（R_3）和100％草（R_4）。通过缓冲溶液和粪便以2：1的比例加入所有处理，以厌氧条件为100ml血清注射瓶。观察到的参数是2,4,6,8,10,12,24和48小时的气体生产，pH和气体动力学（ORSKOV的等式）。结果表明，在观察到的参数上，治疗具有显着影响（P <0.05）。最高的总天然气生产适用于R_2和R_3治疗，总产量为91.17ml和101.17ml，pH（6.62和6.57），最大气体生产（94.03和97.62ml），天然气速度（0.066和0.084ml /小时） 。治疗不存在显着差异。基于观察到的参数和蛋白水解和纤维纤维微生物的潜在可用性选择的沼气引发剂的氨基烃和潜在可用性的微生物的来源是R_2（70％浓缩+ 30％草）。

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