Observation on the interspecific competition between Callyspongia aerizusa sponge and scleractinian coral was done at Pramuka Island, Kepulauan Seribu National Park Jakarta by SCUBA diving. It was observed that Callyspogia aerizusa sponge attached to or grew nearby the scleractinian coral that caused necrosis of the scleractinian polyps. To analyze whether Callyspongia aerizusa sponge released allelochemical that caused the necrosis of scleractinian polyps, 1 liter of seawater in between sponge and coral was taken by syringe equipped with filter paper along with the Callyspongia aerizusa sponge sample. The seawater was extracted with liquid-liquid extraction using ethyl acetate while the sponge sample was extracted with ethyl acetate as well by maceration method. Each extract was dried with vacuum rotary evaporator and analyzed with HPLC (RP column). The result showed that the seawater extract contains four major compounds (at RT 30.52, 35.96, 37.17, and 38.79) that were also found in the sponge extract. This suggests that Callyspongia aerizusa sponge release allelochemicals into sea water to compete and kill the scleractinian coral.
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机译：在Pramuka Island，Kepulauan Seribu National Park Jakarta By Scuba Diving，对Calyspongia Aerizusa海绵和Scleractian Coral之间的间隙竞争的观察。观察到Callyspogia Aerizusa海绵附着在巩膜珊瑚珊瑚附近或增长，导致巩膜外息肉的坏死。为了分析Callyspongia Aerizusa海绵是否发布了患有溶血性息肉的坏死的释放的等位化学，用滤纸配备有滤纸的海绵和珊瑚之间的1升海水，以及Callyspongia Aerizusa海绵样品。使用乙酸乙酯用液 - 液萃取萃取海水，同时用乙酸乙酯萃取海绵样品。用真空旋转蒸发器干燥每种萃取物，并用HPLC（RP柱）分析。结果表明，海水提取物含有四种主要化合物（在水海绵提取物中也发现了四种主要化合物（在室温30.52,35.52,30.96,37.17和38.79）中。这表明将Callyspongia Aerizusa海绵将来自大学释放到海水中，以竞争和杀死巩膜珊瑚珊瑚。
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