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首页> 外文会议>International Symposium on Current Progress in Mathematics and Sciences >Molecular identification of the medicinal herb plant Ruta angustifolia from Lembang Bandung using chloroplast DNA markers
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Molecular identification of the medicinal herb plant Ruta angustifolia from Lembang Bandung using chloroplast DNA markers

机译：使用叶绿体DNA标记，来自Lembang Bandung的药草植物ruta angustifolia的分子鉴定

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Ruta angustifolia is a medicinal plant that comes from Southern Europe and North Africa. Ruta angustifolia, commonly called inggu in Indonesia, has long been trusted and used as a medicine for various diseases. Although it has been traditionally used to treat some diseases, but sufficient information about Ruta angustifolia plant in Indonesia is still very rare, especially related to molecular data (DNA barcode). The purpose of this study is to obtain molecular information about Ruta angustifolia plant in Lembang, Bandung and compared with existing data in the gene bank. The fact is that research on molecular genetics (DNA barcodes) of Ruta angustifolia in Indonesia has not been done. Total genomic DNA was extracted using the DNeasy Plant Mini Kit from QIAGEN. Chloroplast DNA markers used in this study is chloroplast maturase K (matK) dan chloroplast ribulose-1,5-bisphosphate carboxylate (rbcL). Sample then amplified and sequenced. The rbcL gene is successfully amplified but the matK gene is not. The results of rbcL sequence then compared with the data that has been deposited in the gene bank. The technique used is Basic Local Alignment Search Tool (BLAST). Phylogenetic tree than constructed using MEGA7 software. From the phylogenetic tree, it can be seen that Ruta angustifolia's rbcL sequence closer to Ruta graveolens and Ruta chalepensis than other genus such as Murraya, Halfordia, Zanthoxylum, Toddalia, and Sarcomelicope.
机译：Ruta Angustifolia是一种来自欧洲和北非的药用植物。 Ruta Angustifolia通常在印度尼西亚叫做Inggu，长期以来一直被信任并用作各种疾病的药物。虽然传统上用于治疗某些疾病，但是对印度尼西亚的Ruta angustifolia植物的充分信息仍然非常罕见，特别是与分子数据（DNA条形码）有关。本研究的目的是在Lembang，Bandung中获得有关Ruta Angustifolia植物的分子信息，并与基因库中的现有数据进行比较。事实上，对印度尼西亚鲁塔安斯蒂西亚的分子遗传学（DNA条形码）的研究尚未进行。使用来自Qiagen的DNeasy植物迷你试剂盒提取总基因组DNA。本研究中使用的叶绿体DNA标志物是叶绿体型成熟酶K（MATK）DAN叶绿体核糖胺-1,5-二磷酸羧酸盐（RBCL）。然后放大样品并测序。 RBCL基因被成功扩增，但MATK基因不是。然后将RBCL序列的结果与已沉积在基因库中的数据进行比较。使用的技术是基本的本地对准搜索工具（BLAST）。系统发育树比使用Mega7软件构建。从系统发育树上，可以看出，Ruta angustifolia的RBCL序列更接近Ruta Graveolens和Ruta Chalepensis，而不是其他属，如Murraya，Halfordia，Zanthoxylum，Toddalia和Sarcomelicope。

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