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Comparative Leaf and Root Anatomy of Two Dendrobium Species (Orchidaceae) from Different Habitat in Relation to Their Potential Adaptation to Drought

机译:不同栖息地与潜在适应旱灾不同栖息地的两种石斛(蕙兰省)的比较叶和根系解剖

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Dendrobium capra and Dendrobium arcuatum are closely related in phylogeny, but they have very contrasting vegetative morphology and habitats. D. capra is known as a species that is well-adapted to dry lowland teak forest habitat in East Java, where most trees drop their leaves in summer, while D. arcuatum has adapted to mid or high land moist forest at elevation up to 800 m dpl. In order to investigate their potential adaptation to drought stress in the climate change era, we have compared and analyzed the leaf and root anatomical characteristics of both species. Transversal sections were made using hand mini microtome, dehydrated in graded alcohol series and stained with safranin 1 % and fastgreen 1 %. Leaf scraping technique has been used to prepare paradermal sections, and then dehydrated in graded alcohol series and stained with safranin 1 %. Quantitative anatomical characteristics between D. capra and D. arcuatum have been compared using a t-test. The result showed that there were significant differences on anatomical characters between both species. Compared to D. arcuatum, D. capra shows more developed anatomical features for adapting to drought and dry condition. These anatomical features were a thicker cuticle, thicker epidermis, presence of hypodermis, thicker mesophyll, broader primary vascular bundle, well developed xylem's sclerenchyma, lower stomatal density, thicker and high proportion of velamen.
机译:石斛囊和石斛兰鸟在系统发育中密切相关,但它们具有非常鲜明对比的植物形态和栖息地。 D. Capra被称为一种良好适应东爪哇的低地柚木森林栖息地的物种,大多数树木在夏天落下叶子,而D。奥古瓜抑制在高达800的海拔中的中期或高地潮湿森林。 m dpl。为了调查其对气候变化时代的干旱胁迫的潜在适应,我们已经进行了比较和分析了两种物种的叶子和根系解剖学特征。横向部分使用手工迷你切片体进行,在分级醇系列中脱水,并用Safranin染色1%并快速染色1%。叶片刮擦技术已被用于制备帕拉索瓦部分,然后在分级醇系列中脱水并用Safranin 1%染色。 D. Capra和D之间的定量解剖学特征使用T检验进行了比较。结果表明,两种物种之间的解剖结构存在显着差异。与D. arcuatum相比,D. Capra表现出更多开发的解剖功能,以适应干旱和干燥条件。这些解剖学特征是一个较厚的角质层,厚表皮,皮下注射的存在,厚度叶片,更广泛的初级血管束,均匀开发的Xylem的硬化,较低的气孔密度,较厚和Velamen的高比例。

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