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首页> 外文会议>Basic Science International Conference >Genetic Similarity Analysis of Rhynchostylis retusa (L.) Blume Orchids Using OPA 15 and OPA 03 RAPD Marker
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Genetic Similarity Analysis of Rhynchostylis retusa (L.) Blume Orchids Using OPA 15 and OPA 03 RAPD Marker

机译：rhynchostylis retusa（L.）使用OPA 15和OPA 03 RAPD标记的rhynchostylis retusa（L.）的遗传相似性分析

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Rhynchostylis retusa (L.) Blume is a monopodial epiphytic orchid species with attractive flowers arranged in racemose inflorescence. Mass propagation of wild type orchids were generally done by regenerated seeds from the same fruit. The study aimed to know the genetic similarity level of R. retusa plants which are regenerated from the same fruit seeds produced by self-pollination. The seeds were sown on New Phaleonopsis (NP) in vitro culture medium supplemented by 1.5% coconut water (CO). Genetic similarity levels were analyzed using PCR based Random Amplification of DNA Polimorfism (PCR-RAPD) with OPA 15 and OPA 03 primers. The analysis was performed 19 months after sowing the seed (m.a.s). DNA were isolated from 10 plants using a modified Castillo method. The electroforegram of the PCR result was converted to binary data and analyzed using NTSYSpc software version 2.02. The DNA analysis shows that all plants which were tested have a high similarity of DNA character.
机译：Rhynchostylis Retusa（L.）Blume是一种单涂层果皮兰花种类，具有含有吸引力的鲜花，排列在恒星花序中。野生型兰花的大众繁殖通常通过来自同一果实的再生种子来完成。该研究旨在了解R. Retusa植物的遗传相似性水平，其从自授粉产生的同一果子种子中再生。将种子播种在含有1.5％椰子水（CO）的新的phaleoNopsis（NP）中的体外培养基。使用基于PCR的DNA脊髓灰质射出（PCR-RAPD）与OPA 15和OPA 03引物进行遗传相似性水平。播种种子（M.A.S）后19个月进行了分析。使用改性的Castillo方法从10株植物中分离DNA。 PCR结果的电啮合被转换为二进制数据并使用NTSYSPC软件2.02进行分析。 DNA分析表明，测试的所有植物具有高相似性的DNA特征。

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