• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Basic Science International Conference >Methanol Extract of Pomegranate Fruits Containing Ellagic Acid and Cytotoxicity in Vero Cell Line
【24h】

Methanol Extract of Pomegranate Fruits Containing Ellagic Acid and Cytotoxicity in Vero Cell Line

机译：甲醇提取物含有鞣酸和vero细胞系细胞毒性的石榴水果

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Sodium Glucose Co-transporter 2 (SGLT2) inhibitor is an alternative pharmacology therapy for patients with type 2 diabetes mellitus who are unresponsive to metformin as the standard therapy. SGLT2 is a protein transporter that reabsorbs 90% of glucose in the renal tubules. Ellagic acid is found in pomegranate seeds or peels. which potentially could be an SGLT2 inhibitor in silico. This study analyzed the cytotoxicity effect of methanol extract of pomegranate seeds (MPS) and peels (MPP) on the Vero cell line. Laboratory experiments with a post-test only control group design was conducted in this study. Methanol extracts of pomegranate seeds and peels were obtained from our previous study and Dapagliflozin was used as a positive control. The concentrations of ellagic acid in MPS and MPP were measured using liquid chromatography tandem-mass spectrometry (LC-MS/MS). Eight different doses of MPS and MPP, which ranged from 0.98 to 125 ppm, were used to determine their cytotoxicity using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The probit test was then used to analyze IC_(50) of MPS and MPP. The concentration of ellagic acid in MPS was 1.34 ppm, which had IC_(50) at 231 mM, while the concentration of MPP-extracted ellagic acid was 1.68 ppm and had lower IC_(50) (1.7 mM), compared to the IC_(50) of MPS. Moreover, the IC_(50) value of both methanol extracts was higher than the IC_(50) of Dapagliflozin (0.406 mM). In conclusion, MPS and MPP have different cytotoxicity against the Vero cell line.
机译：钠葡萄糖共转运蛋白2（SGLT2）抑制剂是治疗2型糖尿病谁是无反应的二甲双胍作为标准治疗的替代药理学疗法。 SGLT2是一种蛋白质转运是再吸收90％的葡萄糖在肾小管。鞣花酸在石榴籽或果皮中发现。这潜在地可以是在计算机芯片上的SGLT2抑制剂。该研究分析了Vero细胞系石榴籽的甲醇提取物（MPS）和果皮（MPP）的细胞毒性作用。实验室研究与试验后只对照组设计在这项研究中进行。从我们以前的研究获得了石榴籽和皮的甲醇提取物和达格列作为阳性对照。使用液相色谱串联质谱法（LC-MS / MS）测量在MPS和MPP的鞣花酸的浓度。八个不同剂量的MPS和MPP，其范围为0.98〜125ppm的的，用于确定使用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴化物（MTT）测定法它们的细胞毒性。概率单位试验然后用于分析MPS和MPP的IC_（50）。在MPS的鞣花酸的浓度为1.34 ppm的，其在231毫有IC_（50），而MPP-提取鞣花酸的浓度为1.68 ppm或具有较低的IC_（50）（1.7毫摩尔），相对于IC_（ 50）MPS的。而且，无论甲醇提取物的IC_（50）值比达帕格列净（0.406毫摩尔）的IC_（50）更高。总之，MPS和MPP必须对Vero细胞系不同的细胞毒性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号