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首页> 外文会议>Annual Meeting of The National Mastitis Council (U.S.). >Effect of Technique and Timing of Sampling on the Interpretation of Microbiological Results of Bovine Quarter Milk Samples Analyzed withMultiplex Real-time PCR
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Effect of Technique and Timing of Sampling on the Interpretation of Microbiological Results of Bovine Quarter Milk Samples Analyzed withMultiplex Real-time PCR

机译：对牛季牛奶样品微生物样品解释分析的效果及其对牛氨基醛样品的解释分析

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In multiplex real-time PCR specific parts of the genome from different microbial target species are multiplied, quantified and detected simultaneously. In assays developed for diagnosing bovine IMI, PCR test panels include predesigned oligos for mostcommon mastitis causing pathogens (Koskinen et al., 2009). Objectivity, short turn-around times and higher sensitivity have been listed as advantages of PCR over the conventional bacterial culture (BC). PCR amplifies DNA also from dead and growth-inhibited pathogens. BC negative samples often yield positive PCR results (Taponen et al, 2009). This is considered as an advantage in some contexts and as a concern in others. Accepted guidelines for interpretation of PCR results are lacking. The aim of this short review was to highlight factors related to sampling technique and timing of milk sampling, which affect PCR results.
机译：在多重实时PCR特异性部分从不同的微生物靶物种中的基因组进行，同时乘以，量化和检测。在为诊断牛IMI开发的测定中，PCR试验板包括用于雌性乳腺炎的预测寡核苷酸，导致病原体（Koskinen等，2009）。在常规细菌培养（BC）上被列为PCR的优势，差距和更高的敏感性。 PCR也来自死亡和生长抑制病原体的DNA。 BC阴性样品通常产生阳性PCR结果（Taponen等，2009）。这被认为是某些背景下的优势，并作为其他人的担忧。缺乏已接受的解释的解释指南。本次要评审的目的是突出与抽样技术和牛奶抽样时机相关的因素，影响PCR结果。

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