• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>International Conference on Sustainable Energy, Environment and Information Engineering >Bacterial Community Characteristics and Detection of Denitrifying Functional Genes nirS, nirk in the Coastal Water of Bohai Bay, China: Bacterial and denitrifying functional genes in Bohai
【24h】

Bacterial Community Characteristics and Detection of Denitrifying Functional Genes nirS, nirk in the Coastal Water of Bohai Bay, China: Bacterial and denitrifying functional genes in Bohai

机译：渤海湾沿海水中的细菌群落特征及反硝化功能基因NIRS的特征及检测：渤海中的细菌和反硝化功能基因

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To understand the microbial community characteristics and denitrification status in coastal water ecosystem of Bohai Bay, China, the bacterioplankton from six representative stations were collected in September 2016, and the bacterial community and abundance of nir-encoding denitrifying bacteria were studied by 454-pyrosequencing and real-time quantitative PCR (qPCR), respectively. The results showed that the Shannon index of the bacterial community ranged from 4.86 to 5.56. The bacterial composition and their relative abundance varied significantly among the bacterial libraries from the six water samples. Proteobacteria was the largest phylum in the six samples varying between 63.19% and 77.34%. a-proteobacteria was the most abundant class in the W4 station with 46.09%, while y-proteobacteria was the most abundant class in other five stations ranging from 36.39% to 60.58%. The qPCR results showed that the nirS gene abundance ranged from 2.26×10~7 copies/L to 9.63×10~7 copies/L, while nirK gene ranged from 1.01×10~6 copies/L to 2.09×10~7 copies/L, indicating that both of them played important roles during the denitrification of the local coastal water. Furthermore, the nirS abundance in each station was significantly higher than that of nirK, suggesting that the functional genes nirS played more important role than nirK in reduction process of nitrite (NO_2~-) to nitric oxide (NO). Canonical correspondence analysis (CCA) results indicated that petroleum, arsenic (As), chromium (Cr), lead (Pb) and cadmium (Cd) had significant effects on the distribution of bacterial community. In contrast, the key factors regulating the nirS and nirK gene abundances included nitrate (NO_3-N), phosphate (PO_3-P), chlorophyll a (Chla), As and Cd.
机译：要了解中国渤海湾沿海水域生态系统中的微生物群落特征和反硝化现状，于2016年9月收集了六个代表站的菌株，并研究了454-焦点测定的细菌群落和编码脱氮细菌的丰富。实时定量PCR（QPCR）。结果表明，细菌群落的香农指数范围为4.86至5.56。细菌组合物及其相对丰度在六个水样中的细菌文库中显着变化。植物杆菌是六个样品中最大的字段，其含量为63.19％和77.34％。 A-Proteobacteria是W4站中最丰富的课程，46.09％，而Y-植物体外是其他五个站的阶级，范围从36.39％到60.58％。 QPCR结果表明，NIRS基因丰度为2.26×10〜7拷贝/ l至9.63×10〜7份/ l，而NIRK基因范围从1.01×10〜6拷贝/ l到2.09×10〜7份/ L，表明它们两者在当地沿海水的反硝化期间发挥了重要作用。此外，每个车站的NIRS丰富显着高于NIRK，这表明功能基因NIR在亚硝酸盐（NO_2〜 - ）的还原过程中，鼻内的尿液在氮氧化物（NO）中起作用更重要的作用。规范对应分析（CCA）结果表明石油，砷（AS），铬（Cr），铅（Pb）和镉（CD）对细菌群落的分布具有显着影响。相反，调节内联和鼻内基因丰富的关键因素包括硝酸盐（NO_3-N），磷酸盐（PO_3-P），叶绿素A（CHLA），AS和CD。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号