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Substitution VW Medium Using Palm Date Puree in The Tissue Culture of Grammatophyllum Scriptum

机译:使用棕榈枣酱替代vw培养基在语法文体组织培养中

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The research aimed to determine date palm pure concentration and its effect on Tissue Culture of Grammatophyllum scriptum. The research used an experimental method with single factor and seven treatments arranged in a Completely Randomized Design. The treatments were: (1) Vacin & Went (VW) medium; (2) 50g/L date palm puree; (3) 100g/L date palm puree; (4) 150g/L date palm puree; (5) 50g/L date palm puree + 30g/L sugar cane; (6) 100g/L date palm puree + 30g/L sugar cane; (7) 150g/date palm puree L + 30g/L sugar cane. All date palm puree treatments were combined with 3g/L foliar fertilizer. The observed variables were plant heights, number of leaves, number of shoots, the percentage of contamination, browning, life, and rooted explants. The result showed the growth and living performance of G. scriptum in the medium of 50g/L dates palm puree with and without sugar was not significantly different on the VW medium. It indicated that the growth of G. scriptum in this combination of such media was relatively similar with those grown on VW medium. The medium of 50g/L dates palm puree combined with 3g/L foliar fertilizer could substitute the VW medium in the subculture of G. scriptum.
机译:研究旨在确定枣棕榈纯浓度及其对语法文体组织培养的影响。该研究使用了单因素的实验方法,并以完全随机设计排列的七种处理。该治疗方法是:(1)vacin&get(vw)培养基; (2)50g / L日期棕榈酱; (3)100g / l日期棕榈酱; (4)150g / L日期棕榈酱; (5)50g / L日期棕榈酱+ 30g / L甘蔗; (6)100g / L日期棕榈酱+ 30g / L甘蔗; (7)150g / date palm puree l + 30g / l甘蔗。所有日期棕榈酱治疗与3G / L叶面肥料合并。观察到的变量是植物高度,叶子数,芽数,污染,褐变,寿命和生根外植体的百分比。结果表明,VW培养基上的50g / L日期的棕榈酱中的G. Scriptum的生长和生活性能没有显着不同。它表明,在这种介质中,这种介质组合的G. Scriptum的生长与在VW培养基上生长的那些相似。 50g / l日期的棕榈酱与3g / l叶面肥料的介质可以替代VW培养基在G. Scriptum的亚文栽培中。

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