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首页> 外文会议>International Conference on Food and Agriculture Resources >Phospholipase A2-Mediated Preparation of Phosphatidylcholine Containing Ricinoleic Acid and its Anti-inflammatory Effect in vitro
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Phospholipase A2-Mediated Preparation of Phosphatidylcholine Containing Ricinoleic Acid and its Anti-inflammatory Effect in vitro

机译：磷脂酶A2介导的含有蓖麻油酸的磷脂酰胆碱的制备及其在体外抗炎作用

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摘要

Ricinoleic acid (RA) is a type of fatty acid found in castor oil and has been known to have anti-inflammatory effects Phospholipids are useful functional compounds in food/medical fields due to their amphiphilic property and biocompatibility. The purpose of this study was to prepare phospholipid containing RA and to evaluate the compound's antiinflammatory activity in vitro. Lyso-phosphatidylcholine (LPC) from egg yolk was subjected to phospholipase A_2-mediated esterification for the preparation of phosphatidylcholine containing RA (RA-PC). The prepared RA-PC was then evaluated for its anti-inflammatory activity against the murine macrophage-like cell line RAW264.7. Using glycerol as solvent and formamide as water mimic, RA-PC was successfully prepared. Upon optimizing the molar ratio (RA/LPC), the amount of glycerol, and the reaction time, a maximum yield of 57.5 mol% was obtained. A dose dependent decrease in the expression levels of IL-6 and IL-lp was observed upon addition of RA-PC into the culture medium of RAW264.7. Down regulation of gene expression was higher for RA-PC than for RA or Soy-PC, which suggests that the anti-inflammatory effects of RA were improved following phosphatidylation. Our data suggest that RA-PC is a potential compound for use as an antiinflammatory agent.
机译：蓖麻油酸（Ra）是蓖麻油中发现的一种脂肪酸，并且已知由于其两亲性能和生物相容性，磷脂是食品/医学领域中有用的功能性化合物。本研究的目的是制备含有Ra的磷脂并在体外评估化合物的抗炎活性。从蛋黄溶血磷脂酰胆碱（LPC）进行磷脂酶A_2介导的酯化含有RA（RA-PC）的制备磷脂酰胆碱。然后评价制备的RA-PC对鼠巨噬细胞样细胞系Raw264.7的抗炎症活性。使用甘油作为溶剂和甲酰胺作为水模拟，成功制备RA-PC。优化摩尔比（RA / LPC），甘油量和反应时间，获得57.5mol％的最大收率。在加入Ra-PC进入Raw264.7的培养基时，观察到IL-6和IL-LP表达水平的剂量依赖性降低。 RA-PC的基因表达的调节比Ra或大豆PC更高，这表明磷脂酰化后改善了Ra的抗炎作用。我们的数据表明RA-PC是用作抗炎剂的潜在化合物。

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