首页> 外文会议>International Conference on Bioinformatics and Nanomedicine from Natural Resources for Biomedical Research >Isolation and Characterization of Interferon-Induced Feline Tetherin (Bst-2) Protein Expressed by Peripheral Blood Mononuclear Cells (PBMCs)
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Isolation and Characterization of Interferon-Induced Feline Tetherin (Bst-2) Protein Expressed by Peripheral Blood Mononuclear Cells (PBMCs)

机译:外周血单核细胞(PBMCs)表达干扰素诱导的猫蛋白(BST-2)蛋白的分离与表征

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This study was aimed to isolate and characterize the expression of Feline Tetherin/BST-2 protein on PBMCs induced by IFN-a. PBMCs were isolated from blood sample of healthy cats with aged more than 1 year. Samples were divided into two groups, namely the control group and the treatment group which induced by IFN- a at dose of 1000 IU / ml. Total protein was isolated from cultured cells after 24 hours incubations and analyzed using SDS-PAGE, dot blot, and western blotting. The result of SDS-PAGE analysis showed a 35 kDa protein band in treatment group which suggested to be the feline tetherin protein band. The results of dot blot analysis was indicate the positive reaction of antigen Feline Tetherin/ BST-2 and antibody anti BST-2 that marked by purplish blue dot also at treatment group. The western blot analysis showed a confirmation of feline tetherin/BST-2 protein expression with a protein band size approximately 35 kDa molecular weight. Based on the results of this study, can be concluded that administration of IFN-a induction at doses of 1000 IU /ml was able to induce the expression of feline tetherin protein/BST-2 in PBMCs cultured cells, and protein characterization results showed that the feline Tetherin/BST-2 protein has a molecular weight of about 35 kDa.
机译:本研究旨在分离和表征IFN-A诱导的PBMC上的猫西环素/ BST-2蛋白的表达。 PBMCs与1年以上的健康猫的血液样本中分离出来。将样品分为两组,即对照组和由IFN-A诱导的治疗组,其剂量为1000IU / mL。在24小时孵育后,从培养的细胞中分离出总蛋白质,并使用SDS-PAGE,DOT印迹和Western印迹分析。 SDS-PAGE分析的结果显示治疗组中的35kDa蛋白质带,表明是猫西环蛋白蛋白带。点印迹分析的结果表明抗原猫西环素/ BST-2和抗体抗BST-2的正反应,其标记为紫色蓝点也在治疗组上。 Western印迹分析显示猫西环素/ BST-2蛋白表达的确认,蛋白质带尺寸约为35kDa分子量。基于该研究的结果,可以得出结论,给药1000IU / mL时剂量的IFN-A诱导能够诱导PBMCS培养细胞中猫西环蛋白/ BST-2的表达,蛋白表征结果表明猫西环素/ BST-2蛋白的分子量约为35kDa。

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