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Performance of Rhizomucor Miehei in Amidation Reaction for Fatty Monoethanolamide Synthesis Based on Distillated Palm Fatty Acid

机译:基于蒸馏棕榈脂肪酸的脂肪单乙醇酰胺合成的胺化反应中的根瘤菌宫内河的性能

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Lipase is a type of biocatalyst that can work with substrates of lipid classes. Rhizomucor meihei is of lipase enzymes classes, that can react specifically with Palm Fatty Acid Distillate (PFAD) forming fatty monoethanolamide. Monoethanolamide surfactants are compounds which are widely used in the cosmetics industry as emulsifiers. Monoethanolamide having OH-groups, so it is easily soluble in water. Monoethanolamide can be produced from fatty acids with monoethanolamide using alkaline catalyst or a biocatalyst. Production using chemical catalysts will involve high reaction temperature reaches 120°C-150°C, while the reactions involving biocatalysts are like enzyme can be done at a relatively low temperature. This research was conducted by using amination reaction with 3 independent variables i.e. amination temperature at the level of 30°C-70°C, the mole ratio of PFAD: Monetanolamina at (1:1)-(1:20) and ratio of enzyme at 0.05%-0.5% (w/w) and reaction time of 4 hours. The research results show that the Rhizomucor meihei give the best performance at the level of temperature 60°C, ratio mole (1:4) and the ratio of enzyme 0.2% (w/w) with a conversion product of 86.25%.
机译:脂肪酶是一种能够与脂质类衬底合作的生物催化剂。 Rhizomucor Meihei是脂肪酶酶类,可以与棕榈脂肪酸馏出物(PFAD)进行特别反应,形成脂肪单乙醇酰胺。单乙醇酰胺表面活性剂是作为乳化剂的化妆品行业广泛使用的化合物。单乙醇酰胺具有OH-基团,因此它易于溶于水。单乙醇酰胺可以由碱性催化剂或生物催化剂用单乙醇酰胺的脂肪酸制备。使用化学催化剂的生产涉及高反应温度达到120℃-150℃,而涉及生物催化剂的反应就像酶一样,可以在相对低的温度下进行。通过使用3个独立变量的胺化反应进行该研究,即30℃-70℃的胺化温度,PFAD的摩尔比(1:1) - (1:20)和酶的比例和酶的比例在0.05%-0.5%(w / w)和4小时的反应时间。研究结果表明,Rhizomucor Meihei在60°C,比率摩尔(1:4)和酶0.2%(w / w)的比例中具有86.25%的酶0.2%(w / w)的最佳性能。

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