• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>International Conference on Condensed Matters and Advanced Materials >Analysis of Male Urine as Pheromone to Increase Reproduction in Female Tiger Grouper (Epinephelus Fuscoquttatus)
【24h】

Analysis of Male Urine as Pheromone to Increase Reproduction in Female Tiger Grouper (Epinephelus Fuscoquttatus)

机译：雄性尿液作为信息蟹雌性虎斑血管繁殖的分析（Epinephelus Fuscoquttatus）

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The aim of this study was to identify the steroid hormone compound in the urine of male tiger grouper, and the role of steroid hormone suspected as pheromone of the male fish to induce gonad maturity of female tiger grouper. The methods employed in this study included broodstock maintenance, the collection of male urine samples, pheromone analysis of male urine, and application test of urine for gonad maturity of female tiger grouper. The analysis of pheromone content in urine was performed by using LC-MS / MS and FTIR methods. Urine was applied for several females placed in concrete tanks with a volume of 12 tons, 4 females for each tank. The parameters tested were male urine dose: 1 ml, 2 ml, and 3 ml. The results of pheromone analysis using LC-MS / MS method in urine and sperm of male tiger grouper indicated a peak of ion percussion of 255 ms and 273 ms, while the ion products showed 159 F and 255F. Based on the result of FTIR test, it could be seen that the resulted bands were infrared absorption which had certain character patterns, in which urine had six infrared spectrums; this steroid was classified in alcohol group p-sitosterol. The role of urine as a pheromone carrier administered to female was observed through the concentrations of estradiol-17β hormone and testosterone in blood plasma. The results showed that the concentration of estradiol- 17β was much higher when compared with testosterone, either before or after treatment. The high concentration of estradiol was related to the stages of egg development in the female before the spawning season. From the results of this study, it could be concluded that male urine of tiger grouper was a pheromone that could increase the stimulation and gonad maturity of female broodstock.
机译：这项研究的目的是确定男性老虎斑尿中类固醇激素的化合物，并怀疑是雄鱼的信息素类固醇激素的作用，促使女性老虎斑的性腺成熟度。在这项研究中所采用的方法包括亲鱼维护，男性尿液样本的采集，男性尿液中的信息素的分析和应用测试的尿液女性老虎斑的性腺成熟度。通过使用LC-MS / MS和FTIR方法进行的尿中信息素含量的分析。尿涂敷了用于几个女性放置在混凝土罐用12吨，女性为每个槽4的体积。测试的参数为男性小便剂量：1毫升，加入2ml和3ml。信息素的分析用LC-MS / MS方法在尿和雄性老虎斑的精子结果表明的255毫秒和273毫秒离子打击乐器的峰值，而离子产物显示159 F和255F。基于FTIR测试的结果，可以看出，所得到的条带是红外吸收其中有某些字符图形，在该尿有六个红外光谱;这种甾族化合物被归类于醇基团对β-谷甾醇。通过在血液血浆雌二醇17β激素和睾酮的浓度中观察到的尿作为给予雌性信息素载体的作用。结果表明雌二醇的17β浓度高得多的当与睾酮相比，之前或治疗后。雌二醇高浓度的产卵季节之前与卵子发育的各个阶段的女性。从这项研究的结果，可以得出结论，老虎斑的男性尿液是一种信息素，可以增加雌亲鱼的刺激和性腺成熟度。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号